STUDIES ON CONSTITUENTS WITH CYTOTOXIC AND CYTOSTATIC ACTIVITY OF 2 TURKISH MEDICINAL-PLANTS PHLOMIS-ARMENIACA AND SCUTELLARIA-SALVIIFOLIA

Ten known glycosidic compounds, betulalbuside A (1), 8-hydroxylioaloyl,3-O-beta-D-glucopyranoside (2) (monoterpen glycosides), ipolamiide (3) (iridoid glycoside), acteoside (verbascoside) (4), leucosceptoside A (5), martynoside (6), forsythoside B (7), phlinoside B (8), phlinoside C (9), and teucrioside (10) (phenylpropanoid glycosides) mere isolated from methanolic extracts of Phlomis armeniaca and Scutellaria salviifolia (Labiatae). Structure elucidations were carried out using H-1-, C-13-NMR and FAB-MS spectra, as well as chemical evidence. The cytotoxic and cytostatic activities of isolated compounds were investigated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method. Among the glycosides obtained here, caffeic acid-containing phenylpropanoid (or phenethyl alcohol, or phenylethanoid) glycosides were found to show activity against several kinds of cancer cells. However, they didn't affect the growth and viability of primary-cultured rat hepatocytes. Study of the structure-activity relationship indicated that ortho-dihydroxy aromatic systems of phenylpropanoid glycosides are necessary for their cytotoxic and cytostatic activities.