PANCREASTATIN INCREASES CYTOSOLIC CA2+ IN INSULIN SECRETING RINM5F CELLS

被引：24
|
作者：
SANCHEZMARGALET, V
LUCAS, M
GOBERNA, R
机构：
[1] Department of Medical Biochemistry and Molecular Biology, Medical School of Sevilla, Hospital Virgen Macarena, Sevilla
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1992年 / 88卷 / 1-3期
关键词：
PANCREASTATIN; CALCIUM; ATP; ALPHA-ADRENERGIC RECEPTOR BLOCKER; INSULIN SECRETING CELL LINE (RINM5F);
D O I：
10.1016/0303-7207(92)90017-Z
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We have investigated the effect of pancreastatin on cytosolic Ca2+ concentration in the insulin secreting cell line RINm5F. Changes in [Ca2+]i induced by pancreastatin were detected by Fluo-3 fluorescence using both flow cytometry and batch analysis measurements, and turned out to be from 90 to 315 nM equivalent to 80% of that caused by ATP. which increased [Ca2+]i from 90 nM to 400 nM. This effect of pancreastatin did not depend on extracellular calcium and was not mediated by alpha-adrenergic receptors since it was not prevented by the alpha-blocker yohimbine. It is concluded that pancreastatin has a role in the homeostasis of free cytosolic calcium in the insulin secreting cell line Rinm5F.
引用
收藏
页码：129 / 133
页数：5
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