THE TRANSFORMING ACTIVITY OF ACTIVATED G-ALPHA-12

被引：100

作者：

JIANG, HP ^{[1
]}

WU, DQ ^{[1
]}

SIMON, MI ^{[1
]}

机构：

[1] CALTECH,DIV BIOL,147-75,PASADENA,CA 91125

来源：

FEBS LETTERS | 1993年 / 330卷 / 03期

关键词：

G-PROTEIN; TRANSFORMATION; MUTATION;

D O I：

10.1016/0014-5793(93)80896-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heterotrimeric GTP-binding proteins transduce receptor-mediated extracellular signals to their effectors. Several activated mutations of Gsalpha and Gialpha have been shown to be associated with endocrine tumors. In this report, we have evaluated the transforming activity of an putative activated form of Galpha12 subunit. We found that transient expression in NIH3T3 cells of the Galpha12 mutant with substitution of glutamine-229 for leucine could lead to focus formation and that stably transfected NIH3T3 cells could form colonies in soft agar and tumors in nude mice.

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收藏

页码：319 / 322

页数：4

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