FOLDING AND TRIMERIZATION OF CLATHRIN SUBUNITS AT THE TRISKELION HUB

被引：135
作者：
NATHKE, IS
HEUSER, J
LUPAS, A
STOCK, J
TURCK, CW
BRODSKY, FM
机构：
[1] UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM,SAN FRANCISCO,CA 94143
[2] UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL,SAN FRANCISCO,CA 94143
[3] WASHINGTON UNIV,SCH MED,DEPT CELL BIOL,ST LOUIS,MO 63110
[4] PRINCETON UNIV,DEPT MOLEC BIOL,PRINCETON,NJ 08544
[5] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY
[6] UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,SAN FRANCISCO,CA 94143
[7] UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143
来源：
CELL | 1992年 / 68卷 / 05期
关键词：
D O I：
10.1016/0092-8674(92)90033-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The triskelion shape of the clathrin molecule enables it to form the polyhedral protein network that covers clathrin-coated pits and vesicles. Domains within the clathrin heavy chain that are responsible for maintaining triskelion shape and function were identified and localized. Sequences that mediate trimerization are distal to the carboxyl terminus and are adjacent to a domain that mediates both light chain binding and clathrin assembly. Structural modeling predicts that within this domain, the region of heavy chain-light chain interaction is a bundle of three or four a helices. These studies establish a low resolution model of clathrin subunit folding in the central portion (hub) of the triskelion, thus providing a basis for future mutagenesis experiments.
引用
收藏
页码：899 / 910
页数：12
相关论文
共 45 条
[21]  
JACKSON AP, 1988, J BIOL CHEM, V263, P16688
[22]   CLATHRIN LIGHT-CHAINS CONTAIN BRAIN-SPECIFIC INSERTION SEQUENCES AND A REGION OF HOMOLOGY WITH INTERMEDIATE FILAMENTS [J].
JACKSON, AP ;
SEOW, HF ;
HOLMES, N ;
DRICKAMER, K ;
PARHAM, P .
NATURE, 1987, 326 (6109) :154-159
[23]   CLATHRIN AND ASSOCIATED ASSEMBLY AND DISASSEMBLY PROTEINS [J].
KEEN, JH .
ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :415-438
[24]   CLATHRIN-COATED VESICLES - ISOLATION, DISSOCIATION AND FACTOR-DEPENDENT REASSOCIATION OF CLATHRIN BASKETS [J].
KEEN, JH ;
WILLINGHAM, MC ;
PASTAN, IH .
CELL, 1979, 16 (02) :303-312
[25]   CLATHRIN LIGHT-CHAINS LCA AND LCB ARE SIMILAR, POLYMORPHIC, AND SHARE REPEATED HEPTAD MOTIFS [J].
KIRCHHAUSEN, T ;
SCARMATO, P ;
HARRISON, SC ;
MONROE, JJ ;
CHOW, EP ;
MATTALIANO, RJ ;
RAMACHANDRAN, KL ;
SMART, JE ;
AHN, AH ;
BROSIUS, J .
SCIENCE, 1987, 236 (4799) :320-324
[26]   CONFIGURATION OF CLATHRIN TRIMERS - EVIDENCE FROM ELECTRON-MICROSCOPY [J].
KIRCHHAUSEN, T ;
HARRISON, SC ;
HEUSER, J .
JOURNAL OF ULTRASTRUCTURE AND MOLECULAR STRUCTURE RESEARCH, 1986, 94 (03) :199-208
[27]   LOCATION AND DISTRIBUTION OF THE LIGHT-CHAINS IN CLATHRIN TRIMERS [J].
KIRCHHAUSEN, T ;
HARRISON, SC ;
PARHAM, P ;
BRODSKY, FM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (09) :2481-2485
[28]   STRUCTURAL DOMAINS OF CLATHRIN HEAVY-CHAINS [J].
KIRCHHAUSEN, T ;
HARRISON, SC .
JOURNAL OF CELL BIOLOGY, 1984, 99 (05) :1725-1734
[29]   CLATHRIN HEAVY-CHAIN - MOLECULAR-CLONING AND COMPLETE PRIMARY STRUCTURE [J].
KIRCHHAUSEN, T ;
HARRISON, SC ;
CHOW, EP ;
MATTALIANO, RJ ;
RAMACHANDRAN, KL ;
SMART, J ;
BROSIUS, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) :8805-8809
[30]   CHARACTERISTICS OF THE TYROSINE RECOGNITION SIGNAL FOR INTERNALIZATION OF TRANSMEMBRANE SURFACE GLYCOPROTEINS [J].
KTISTAKIS, NT ;
THOMAS, D ;
ROTH, MG .
JOURNAL OF CELL BIOLOGY, 1990, 111 (04) :1393-1407
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