THE 3'-TERMINAL REGION OF THE HYGROMYCIN-B-RESISTANCE GENE IS IMPORTANT FOR ITS ACTIVITY IN ESCHERICHIA-COLI AND NICOTIANA-TABACUM

被引：87

作者：

BILANG, R ^{[1
]}

IIDA, S ^{[1
]}

PETERHANS, A ^{[1
]}

POTRYKUS, I ^{[1
]}

PASZKOWSKI, J ^{[1
]}

机构：

[1] SCI UNIV TOKYO, DEPT BIOL SCI & TECHNOL, NODA, CHIBA 278, JAPAN

来源：

GENE | 1991年 / 100卷

基金：

日本学术振兴会;

关键词：

RECOMBINANT DNA; GENE EXPRESSION; SELECTIVE MARKER; PLANT TRANSFORMATION; C-TERMINUS; PHOSPHOTRANSFERASE;

D O I：

10.1016/0378-1119(91)90375-L

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have modified the 3'-coding region of the hygromycin B (Hy) phosphotransferase-encoding gene (hph). The level of Hy resistance conferred by the modified hph genes was determined in Escherichia coli and Nicotiana tabacum. Polypeptides up to 12 amino acids shorter or longer than the wild-type (wt) protein remained active in both pro- and eukaryotic cells. A gene construct of wt length, but with a modified 3' sequence, was less active as measured by the level of Hy resistance. Therefore, the nature of the C terminus of the hph gene product, rather than the length of the polypeptide, influences the ability to confer resistance to Hy.

引用

页码：247 / 250

页数：4

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