LOCALIZATION OF LOW-MOLECULAR-WEIGHT GTP BINDING-PROTEINS TO EXOCYTIC AND ENDOCYTIC COMPARTMENTS

被引：1039

作者：

CHAVRIER, P ^{[1
]}

PARTON, RG ^{[1
]}

HAURI, HP ^{[1
]}

SIMONS, K ^{[1
]}

ZERIAL, M ^{[1
]}

机构：

[1] BIOZENTRUM,DEPT PHARMACOL,CH-4056 BASEL,SWITZERLAND

来源：

CELL | 1990年 / 62卷 / 02期

关键词：

D O I：

10.1016/0092-8674(90)90369-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of 11 clones encoding putative GTP binding proteins highly homologous to the yeast YPT1 SEC4 gene products have been isolated from an MDCK cell cDNA library. We localized three of the corresponding proteins in mammalian cells by using affinity-purified antibodies in immunofluorescence and immunoelectron microscopy studies. One, the MDCK homolog of rab2, is associated with a structure having the characteristics of an intermediate compartment between the endoplasmic reticulum and the Golgi apparatus. The second, rab5, is located at the cytoplasmic surface of the plasma membrane and on early endosomes, while the third, rab7, is found on late endosomes. These findings provide evidence that members of the YPT1 SEC4 subfamily of GTP binding proteins are localized to specific exocytic and endocytic subcompartments in mammalian cells. © 1990.

引用

页码：317 / 329

页数：13

共 62 条

[1] THE SUBCELLULAR ORGANIZATION OF MADIN-DARBY CANINE KIDNEY-CELLS DURING THE FORMATION OF A POLARIZED EPITHELIUM [J].