ANALYSIS OF CYCLIC NUCLEOTIDE-RELATED ENZYMES BY CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY

被引：8
作者：
LANGRIDGE, JI
BRENTON, AG
WALTON, TJ
HARRIS, FM
NEWTON, RP
机构：
[1] UNIV COLL SWANSEA,SCH BIOL SCI,BIOCHEM RES GRP,SWANSEA SA2 8PP,WALES
[2] UNIV COLL SWANSEA,DEPT CHEM,MASS SPECTROMETRY RES UNIT,SWANSEA SA2 8PP,WALES
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1993年 / 7卷 / 04期
关键词：
D O I：
10.1002/rcm.1290070407
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Continuous-flow fast-atom bombardment mass spectrometry has been developed to directly monitor cyclic nucleotide (substrate) and its product levels from an on-going phosphodiesterase reaction. Analysis of cAMP and cCMP phosphodiesterase incubates have been performed where the temporal evolution of the enzymic reaction is monitored and the effect of enzyme concentration upon the rate of reaction determined. Quantitative data on the enzyme kinetics have been obtained, in the form of Lineweaver-Burke plots, that are shown to correlate well with well-established radiometric methods.
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页码：293 / 303
页数：11
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