UP-REGULATION OF FORMYL-PEPTIDE AND INTERLEUKIN-8-INDUCED EOSINOPHIL CHEMOTAXIS IN PATIENTS WITH ALLERGIC-ASTHMA

被引:111
作者
WARRINGA, RAJ
MENGELERS, HJJ
RAAIJMAKERS, JAM
BRUIJNZEEL, PLB
KOENDERMAN, L
机构
[1] UNIV HOSP UTRECHT,DEPT PULM DIS,POB 85500,3500 GA UTRECHT,NETHERLANDS
[2] CENT MIL HOSP UTRECHT,UTRECHT,NETHERLANDS
[3] NETHERLANDS ORG APPL SCI RES,MED BIOL LAB,RIJSWIJK,NETHERLANDS
关键词
ASTHMA; ALLERGEN PROVOCATION; CHEMOTAXIS; HUMAN EOSINOPHILS;
D O I
10.1016/0091-6749(93)90323-8
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: The cytokine granulocyte-macrophage colony-stimulating factors, interleukin-3 and interleukin-5, are important modulators of eosinophilia and eosinophil function. In particular, eosinophil chemotaxis is very sensitive to cytokine priming. Methods: We evaluated chemotactic responses of eosinophils from Patients with allergic asthma. These cells exhibited a primed phenotype as deduced from enhanced responses toward formyl-methionyl-leucyl-phenylalanine and platelet-activating factor and a decreased responsiveness toward granulocyte-macrophage colony-stimulating factor. Bronchoprovocation of patients with allergic asthma with allergen was performed as a possible means to enhance in vivo priming. Results: Indeed, eosinophils isolated 3 hours after allergen challenge exhibited a more pronounced primed phenotype, which was reflected by an induction of responsiveness towards interleukin-& Eosinophil responses induced by platelet-activating factor, formyl-methionyl-leucyl-phenylalanine, complement fragment C5a, interleukin-3, interleukin-5, and granulocyte-macrophage colony-stimulating factor were not significantly altered after allergen challenge. Conclusion: These data provide further evidence that eosinophils are already primed in the peripheral blood of individuals with allergic asthma. This is most likely due to the presence of circulating cytokines in the peripheral blood of those individuals. This in vivo priming results in selective upregulation and downregulation of responses toward various chemotaxins, which may be released in the lungs during allergic inflammation.
引用
收藏
页码:1198 / 1205
页数:8
相关论文
共 40 条
[1]   DISSIMILARITY IN METHACHOLINE AND ADENOSINE 5'-MONOPHOSPHATE RESPONSIVENESS 3-H AND 24-H AFTER ALLERGEN CHALLENGE [J].
AALBERS, R ;
KAUFFMAN, HF ;
KOETER, GH ;
POSTMA, DS ;
DEVRIES, K ;
DEMONCHY, JGR .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1991, 144 (02) :352-357
[2]   EFFECTS OF CORTICOSTEROIDS ON EOSINOPHIL CHEMOTAXIS AND ADHERENCE [J].
ALTMAN, LC ;
HILL, JS ;
HAIRFIELD, WM ;
MULLARKEY, MF .
JOURNAL OF CLINICAL INVESTIGATION, 1981, 67 (01) :28-36
[3]  
ATKINS PC, 1983, CLIN REV ALLERG, V1, P385
[4]   IDENTIFICATION OF ACTIVATED LYMPHOCYTES-T AND EOSINOPHILS IN BRONCHIAL BIOPSIES IN STABLE ATOPIC ASTHMA [J].
AZZAWI, M ;
BRADLEY, B ;
JEFFERY, PK ;
FREW, AJ ;
WARDLAW, AJ ;
KNOWLES, G ;
ASSOUFI, B ;
COLLINS, JV ;
DURHAM, S ;
KAY, AB .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1990, 142 (06) :1407-1413
[5]   NEUTROPHIL-ACTIVATING PEPTIDE-1 INTERLEUKIN-8, A NOVEL CYTOKINE THAT ACTIVATES NEUTROPHILS [J].
BAGGIOLINI, M ;
WALZ, A ;
KUNKEL, SL .
JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (04) :1045-1049
[6]   ADHESION OF HUMAN BASOPHILS, EOSINOPHILS, AND NEUTROPHILS TO INTERLEUKIN 1-ACTIVATED HUMAN VASCULAR ENDOTHELIAL-CELLS - CONTRIBUTIONS OF ENDOTHELIAL-CELL ADHESION MOLECULES [J].
BOCHNER, BS ;
LUSCINSKAS, FW ;
GIMBRONE, MA ;
NEWMAN, W ;
STERBINSKY, SA ;
DERSEANTHONY, CP ;
KLUNK, D ;
SCHLEIMER, RP .
JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 173 (06) :1553-1556
[7]   EOSINOPHILIC INFLAMMATION IN ASTHMA [J].
BOUSQUET, J ;
CHANEZ, P ;
LACOSTE, JY ;
BARNEON, G ;
GHAVANIAN, N ;
ENANDER, I ;
VENGE, P ;
AHLSTEDT, S ;
SIMONYLAFONTAINE, J ;
GODARD, P ;
MICHEL, FB .
NEW ENGLAND JOURNAL OF MEDICINE, 1990, 323 (15) :1033-1039
[9]   INHIBITION OF NEUTROPHIL AND EOSINOPHIL INDUCED CHEMOTAXIS BY NEDOCROMIL SODIUM AND SODIUM CROMOGLYCATE [J].
BRUIJNZEEL, PLB ;
WARRINGA, RAJ ;
KOK, PTM ;
KREUKNIET, J .
BRITISH JOURNAL OF PHARMACOLOGY, 1990, 99 (04) :798-802
[10]   THE RELEASE OF PLATELET-ACTIVATING-FACTOR INTO PLASMA DURING ALLERGEN-INDUCED BRONCHOCONSTRICTION [J].
CHANYEUNG, M ;
LAM, S ;
CHAN, H ;
TSE, KS ;
SALARI, H .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1991, 87 (03) :667-673