CHARACTERIZATION OF AN EXTRACELLULAR BETA-1,3-GLUCANASE OF CLAVICEPS-PURPUREA

被引：14

作者：

BROCKMANN, B ^{[1
]}

SMIT, R ^{[1
]}

TUDZYNSKI, P ^{[1
]}

机构：

[1] UNIV MUNSTER, INST BOT, SCHLOSSGARTEN 3, W-4400 MUNSTER, GERMANY

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 1992年 / 40卷 / 03期

关键词：

D O I：

10.1016/0885-5765(92)90060-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The phytopathogenic fungus Claviceps purpurea produces an extracellular β-1,3-glucanase in axenic culture. The enzyme was purified and characterized. It is a glycoprotein with a pH optimum of 4·5, a molecular weight of about 90 kDa, and an isoelectric point of 9·0. The latter two properties are unusual for a fungal β-1,3-glucanase. Analysis of substrate specificity confirmed that the enzyme is a specific endo-β-1,3-glucanase. A comparable enzyme was also detected in honeydew of infected plants. A possible role for this enzyme in host-parasite interaction (degradation of callose protecting the host's vascular tissue) is discussed. © 1992.

引用

页码：191 / 201

页数：11

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