THIOREDOXIN REDUCTION DEPENDENT ON ALPHA-KETOACID OXIDATION BY ALPHA-KETOACID DEHYDROGENASE COMPLEXES

被引：36

作者：

BUNIK, V ^{[1
]}

FOLLMANN, H ^{[1
]}

机构：

[1] UNIV KASSEL, FACHBEREICH BIOL CHEM, D-34109 KASSEL, GERMANY

来源：

FEBS LETTERS | 1993年 / 336卷 / 02期

关键词：

THIOREDOXIN; THIOREDOXIN REDUCTASE; ALPHA-KETOACID DEHYDROGENASE COMPLEX; INSULIN; LIPOATE; MITOCHONDRION;

D O I：

10.1016/0014-5793(93)80801-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pyruvate and alpha-ketoglutarate dehydrogenase complexes isolated from pig heart mitochondria promote the reduction of thioredoxin in the presence of their alpha-ketoacid substrates, coenzyme A, and free lipoate. Substrate-specific generation of reduced thioredoxin was established by two independent methods, viz. reduction of insulin and thioredoxin reductase-catalyzed NADPH formation. Dihydrolipoate accumulating in the absence of NAD+ is the likely intermediate. A redox function in alpha-ketoacid oxidation provides a potential role for the specific thioredoxins previously identified by us in mitochondria.

引用

页码：197 / 200

页数：4

共 12 条

[1] EFFECTS OF DISULFIDES AND ALPHA-OXOGLUTARATE ON NUCLEAR THIOL FORMATION AND THIOL CONTENT OF CHROMATIN IN LYSED RAT SPLEEN NUCLEI [J].

BITNYSZLACHTO, S ;

OCHALSKACZEPULIS, M .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1978, 9 (03) :179-183

[2]

BODENSTEIN J, 1991, Z NATURFORSCH C, V46, P270

[3] ANIMAL AND PLANT-MITOCHONDRIA CONTAIN SPECIFIC THIOREDOXINS [J].

BODENSTEINLANG, J ;

BUCH, A ;

FOLLMANN, H .

FEBS LETTERS, 1989, 258 (01) :22-26

[4]

BORBE H, 1989, NEUE BIOCH PHARMAKOL

[5]

BUNIK VI, 1990, BIOCHEM INT, V22, P967

[6]

HOLMGREN A, 1979, J BIOL CHEM, V254, P9627

[7]

HOLMGREN A, 1989, J BIOL CHEM, V264, P13963

[8] STUDIES ON MECHANISM AND KINETICS OF 2-OXOGLUTARATE DEHYDROGENASE SYSTEM FROM PIG HEART [J].

MCMINN, CL ;

OTTAWAY, JH .

BIOCHEMICAL JOURNAL, 1977, 161 (03) :569-581

[9]

MOORE EC, 1964, J BIOL CHEM, V239, P3453

[10] PURIFICATION OF 2-OXO ACID DEHYDROGENASE MULTI-ENZYME COMPLEXES FROM OX HEART BY A NEW METHOD [J].

STANLEY, CJ ;

PERHAM, RN .

BIOCHEMICAL JOURNAL, 1980, 191 (01) :147-154

← 1 2 →