APPLICATION TO PLANT-PROTEINS OF GEL-ELECTROPHORETIC METHODS

被引：19
作者：
MICHAUD, D
ASSELIN, A
机构：
[1] Département de Phytologie, Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval
来源：
JOURNAL OF CHROMATOGRAPHY A | 1995年 / 698卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(94)00880-I
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The present review describes the major extraction and gel electrophoretic procedures available for the study of plant proteins. Firstly, some technical considerations are made about the extraction of plant proteins, which necessitates special precautions due to the presence in plant cells of several substances that may readily and severely alter the integrity of the proteins extracted. After a brief discussion on the utility of gel electrophoresis for the study of plant enzymes involved in plant growth and development, some of the most useful techniques available to study plant defense proteins are then described. Techniques allowing the specific detection of hydrolases acting on microbial cell walls, in particular, are described in detail. The detection on gels of some inhibitors involved in the resistance of plants to insect pests is also considered.
引用
收藏
页码：263 / 279
页数：17
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