FACTORS INFLUENCING THE DETERMINATION OF MICROBIAL BIOMASS SULFUR IN SOIL

被引:8
作者
BANERJEE, MR
CHAPMAN, SJ
KILLHAM, K
机构
[1] Department of Plant and Soil Science, Aberdeen University, Aberdeen, Scotland, AB92UE, Meston Building
[2] Macaulay Land Use Research Institute, Cragiebuckler, Aberdeen
关键词
D O I
10.1080/00103629309368850
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A laboratory study was carried out to investigate the factors affecting the determination of microbial biomass sulphur (S) in soil in order to develop a more reliable assay. Biomass-S concentrations were determined by chloroform fumigation/direct extraction (with determination of the ks calibration constant using S-35 labelled microorganisms). The effects of period of fumigation, the need for chloroform evacuation, the type of extractant, and the time of extraction were investigated. The optimum values for biomass-S were obtained using a five day fumigation period without chloroform evacuation (use of S-35 labelled microorganisms demonstrated that there is a vacuum sensitive non-biomass-S pool), with CaCl2 as an extractant over a one hour extraction period.
引用
收藏
页码:939 / 950
页数:12
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