Selective Assay for LPS with Polylysine-Immobilized Cellulose Beads and Limulus Amebocyte Lysate

被引：0
|
作者：
Sakata, Masayo [1 ]
Fukuma, Yuriko [1 ]
Kunitake, Masashi [1 ]
Todokoro, Masami [2 ]
机构：
[1] Kumamoto Univ, Grad Sch Sci & Technol, 2-39-1 Kurokami, Kumamoto 8608555, Japan
[2] Chisso Co Ltd, Chiyoda Ku, Tokyo 1008105, Japan
来源：
E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY | 2009年 / 7卷
关键词：
Surface chemical reaction; Adsorption kinetics; Endotoxin assay; LAL-inhibiting substance;
D O I：
10.1380/ejssnt.2009.747
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
We developed a endotoxin ( lipopolysaccharide; LPS) assay using poly (epsilon-lysine)-immobilized cellulose beads and Limulus amebocyte lysate (LAL). This method is composed of two steps. First, endotoxins are selectively adsorbed on the beads in protein solutions, and then the adsorbed endotoxins are separated from the compound such as protein by centrifugation. Second, the endotoxins adsorbed on the beads are directly reacted with the LAL reagent by tubidimetric time assay using Toxinometer ET-2000 (Wako). This assay method could be used widely as a means of assaying LPS in solutions containing LAL-inhibiting or enhancing substances, such as protein.
引用
收藏
页码：747 / 749
页数：3
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