EXPRESSION CLONING AND CHARACTERIZATION OF THE TGF-BETA TYPE-III RECEPTOR

被引：588

作者：

WANG, XF ^{[1
]}

LIN, HY ^{[1
]}

NGEATON, E ^{[1
]}

DOWNWARD, J ^{[1
]}

LODISH, HF ^{[1
]}

WEINBERG, RA ^{[1
]}

机构：

[1] MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

来源：

CELL | 1991年 / 67卷 / 04期

关键词：

D O I：

10.1016/0092-8674(91)90074-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta-1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.

引用

页码：797 / 805

页数：9

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