TETRABORATE CONCENTRATION ON MORGAN-ELSON REACTION AND AN IMPROVED METHOD FOR HEXOSAMINE DETERMINATION

Effect of tetraborate concentration on the determination of hexosamine by Morgan-Elson color reaction according to Levvy and McAllan [(1959) Biochem. J., 73, 127-232] was studied using the three different preparations of the tetraborate solution. The concentration of the previous report during the chromogen formation (0.29 m) was too high comparing to the optimum concentration of the present experiment (0.175 m). An improved method is now proposed which is excellent on the ground of convenience and reproducibility; same readings were obtained with the tetraborate solution from various preparations which were stable at least for two years. It showed same molar extinction coefficient as obtained before. © 1979.