ACTIVATED HUMAN BASOPHILS CONTAIN HISTAMINE IN CYTOPLASMIC VESICLES

被引：26
作者：
DVORAK, AM
MORGAN, ES
LICHTENSTEIN, LM
MACGLASHAN, DW
机构：
[1] HARVARD UNIV, SCH MED, DEPT PATHOL, BOSTON, MA 02115 USA
[2] JOHNS HOPKINS ASTHMA & ALLERGY CTR, DIV CLIN IMMUNOL & ALLERGY, BALTIMORE, MD USA
来源：
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 1994年 / 105卷 / 01期
关键词：
BASOPHIL; MAST CELL; PIECEMEAL DEGRANULATION; HISTAMINE; VESICULAR TRANSPORT; DIAMINE OXIDASE; ULTRASTRUCTURAL ENZYME-AFFINITY GOLD TECHNIQUE;
D O I：
10.1159/000236796
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Human basophils in contact allergy empty granules slowly - a secretory process termed piecemeal degranulation in contrast to the explosive extrusion of granules seen in anaphylactic degranulation. An ultrastructural enzyme-affinity method to detect histamine, employing diamine oxidase coupled to gold, was used to localize this proinflammatory mediator in human basophils stimulated to release histamine by FMLP. Cytoplasmic vesicles and granules of human basophils so stimulated for 20 s were gold-labeled, indicating the presence of histamine. Cytoplasmic vesicles may play a key role in the secretion of histamine from basophils in those diseases characterized by the ultrastructural morphology of piecemeal degranulation.
引用
收藏
页码：8 / 11
页数：4
相关论文
共 29 条
[11]  
DVORAK AM, 1985, AM J PATHOL, V118, P425
[12]   DIAMINE OXIDASE GOLD LABELS HISTAMINE IN HUMAN MAST-CELL GRANULES - A NEW ENZYME-AFFINITY ULTRASTRUCTURAL METHOD [J].
DVORAK, AM ;
MORGAN, ES ;
SCHLEIMER, RP ;
LICHTENSTEIN, LM .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1993, 41 (06) :787-800
[13]  
DVORAK AM, 1992, AM J PATHOL, V141, P1309
[14]  
DVORAK AM, 1981, LAB INVEST, V44, P174
[15]  
DVORAK AM, 1991, BASOPHIL MAST CELL D, V4, P415
[16]  
Dvorak AM, 1992, GRANULOCYTE RESPONSE, P169
[17]  
DVORAK HF, 1974, LAB INVEST, V31, P111
[18]  
DVORAK HF, 1975, CLIN HAEMATOL, V4, P651
[19]  
EHRICH WE, 1953, SCIENCE, V118, P603
[20]  
KAPLAN AP, 1991, ADV IMMUNOL, V50, P237
123