RESIALYLATION OF SIALIDASE-TREATED SHEEP AND HUMAN ERYTHROCYTES BY TRYPANOSOMA-CRUZI TRANS-SIALIDASE - RESTORATION OF COMPLEMENT RESISTANCE OF DESIALYLATED SHEEP ERYTHROCYTES

Trypanosoma cruzi trans-sialidase (TS) is a recently described enzyme which transfers alpha(2-3)-linked sialic acid from host-derived sialylated glycoconjugates to parasite surface molecules [Schenkman et al. (1991) Cell, 65, 1117]. We report here on the ability of TS to transfer sialic acid from donor sialyl-alpha(2-3)lactose to sialidase-treated sheep and human erythrocytes. Up to approximately 50% resialylation of both desialylated red cells could be attained. Resialylation of desialylated sheep erythrocytes restores their resistance to lysis by human complement. This ascribes a possible biological role for T.cruzi TS and demonstrates directly that sialic acid is solely responsible for preventing alternative pathway activation of human complement by sheep erythrocytes.