A MULTIFUNCTIONAL VECTOR FAMILY FOR GENE-EXPRESSION IN MAMMALIAN-CELLS

被引：30

作者：

DIRKS, W

SCHAPER, F

KIRCHHOFF, S

MORELLE, C

HAUSER, H

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,GENET EUKARYOTES,D-38124 BRAUNSCHWEIG,GERMANY

[2] DSM,GERMAN COLLECT MICROORGANISMS & CELL CULTURES,DEPT HUMAN & ANIM CELL CULTURE,D-38124 BRAUNSCHWEIG,GERMANY

[3] SOLVAY SA,CENT LAB,B-1120 BRUSSELS,BELGIUM

来源：

GENE | 1994年 / 149卷 / 02期

关键词：

DICISTRONIC EXPRESSION; IN VITRO TRANSCRIPTION; MAMMALIAN EXPRESSION VECTOR; MPSV-LTR; POLIOVIRUS; RECOMBINANT DNA; T7; PROMOTER; T3;

D O I：

10.1016/0378-1119(94)90186-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A family of different promoters and Vector backbones for multiple use in mammalian cells in vivo and in vitro experiments has been created. The elements allow high-level constitutive, as well as adjustable expression with mono- and dicistronic transcription units and several in vitro manipulations of the inserted ORFs. The vector components are designed to be combined in a simple cloning step.

引用

页码：387 / 388

页数：2

共 7 条

[1] EXPRESSION OF HUMAN BONE MORPHOGENETIC PROTEINS-2 OR PROTEINS-4 IN MURINE MESENCHYMAL PROGENITOR C3H10T1/2 CELLS INDUCES DIFFERENTIATION INTO DISTINCT MESENCHYMAL CELL LINEAGES [J].