RETICULOCYTE MEASUREMENTS AS A BIOASSAY FOR ERYTHROPOIETIN

被引：9

作者：

BARBONE, AG ^{[1
]}

APARICIO, B ^{[1
]}

ANDERSON, DW ^{[1
]}

NATARAJAN, J ^{[1
]}

RITCHIE, DM ^{[1
]}

机构：

[1] RW JOHNSON PHARMACEUT RES INST,LA JOLLA,CA 92037

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1994年 / 12卷 / 04期

关键词：

RETICULOCYTE; ERYTHROPOIETIN; FLOW CYTOMETRY; BIOASSAY;

D O I：

10.1016/0731-7085(93)E0006-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The possibility of using reticulocyte counts in peripheral blood to assay erythropoietic activity of recombinant human erythropoietin (r-HuEPO) was evaluated in normal mice. Mice were injected subcutaneously with r-HuEPO on days 0, 1 and 2 and bled on day 4 for reticulocyte count determinations, using an automated counting system with thiazole orange fluorescent staining and flow cytometric analysis. Reticulocyte counts increased in a dose-dependent fashion upon administration of r-HuEPO. The reticulocyte count was unaffected by asialylated EPO as well as other substances tested (interleukin-1, interleukin-3, dexamethasone, human chorionic gonadotropin). These data demonstrate the usefulness of employing reticulocyte counts as a rapid, specific and reproducible assay for in vivo erythropoietic activity of r-HuEPO.

引用

页码：515 / 522

页数：8

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