DECARBOXYLATION OF BRANCHED-CHAIN ALPHA-KETOACIDS IN HEPATOCYTES FROM ALLOXAN-DIABETIC RATS - THE EFFECT OF INSULIN

被引：1

作者：

STERNICZUK, A ^{[1
]}

WALAJTYSRODE, EI ^{[1
]}

WOJTCZAK, AB ^{[1
]}

机构：

[1] M NENCKI INST EXPTL BIOL,DEPT CELLULAR BIOCHEM,PASTEURA 3,PL-02093 WARSAW,POLAND

来源：

CELL BIOCHEMISTRY AND FUNCTION | 1991年 / 9卷 / 01期

关键词：

BRANCHED-CHAIN KETOACIDS; DIABETES; INSULIN; HEPATOCYTES;

D O I：

10.1002/cbf.290090104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The flux through branched-chain alpha-ketoacid dehydrogenase and the activity of the branched-chain alpha-ketoacid dehydrogenase complex were measured in hepatocytes isolated from fed, starved and alloxan diabetic rats. The highest rate of branched-chain alpha-ketoacid oxidation was found in hepatocytes isolated from starved rats, slightly lower in those from fed rats, and significantly lower in diabetic hepatocytes. The amount of the active form of branched-chain alpha-ketoacid dehydrogenase was only slightly diminished in diabetic hepatocytes, whereas the flux through the dehydrogenase was inversely correlated with the rate of endogenous ketogenesis. The same was observed in hepatocytes isolated from starved rats when branched-chain alpha-ketoacid oxidation was measured in the presence of added oleate. In both cases the diminished flux through the dehydrogenase, restored by a short preincubation of hepatocytes with insulin, was parallelled by a decrease of fatty acid-derived ketogenesis. The significance of these findings is discussed in relation to the role of insulin in branched-chain alpha-ketoacid oxidation in liver of diabetic rats.

引用

页码：13 / 21

页数：9

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