EFFICIENT OLIGONUCLEOTIDE-DIRECTED CONSTRUCTION OF MUTATIONS IN EXPRESSION VECTORS BY THE GAPPED DUPLEX DNA METHOD USING ALTERNATING SELECTABLE MARKERS

被引：308

作者：

STANSSENS, P ^{[1
]}

OPSOMER, C ^{[1
]}

MCKEOWN, YM ^{[1
]}

KRAMER, W ^{[1
]}

ZABEAU, M ^{[1
]}

FRITZ, HJ ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,ZELLBIOL ABT,D-8033 MARTINSRIED,FED REP GER

来源：

NUCLEIC ACIDS RESEARCH | 1989年 / 17卷 / 12期

关键词：

D O I：

10.1093/nar/17.12.4441

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：4441 / 4454

页数：14

共 35 条

[1] MAXIMIZING GENE-EXPRESSION ON A PLASMID USING RECOMBINATION INVITRO [J].

BACKMAN, K ;

PTASHNE, M .

CELL, 1978, 13 (01) :65-71

[2] DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].

BEAUCAGE, SL ;

CARUTHERS, MH .

TETRAHEDRON LETTERS, 1981, 22 (20) :1859-1862

[3] CONSTRUCTION OF PLASMID CLONING VEHICLES THAT PROMOTE GENE-EXPRESSION FROM THE BACTERIOPHAGE LAMBDA-PL PROMOTER [J].

BERNARD, HU ;

REMAUT, E ;

HERSHFIELD, MV ;

DAS, HK ;

HELINSKI, DR .

GENE, 1979, 5 (01) :59-76

[4] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .3. DERIVATIVES OF PLASMID PBR322 CARRYING UNIQUE ECO RI SITES FOR SELECTION OF ECO RI GENERATED RECOMBINANT DNA-MOLECULES [J].

BOLIVAR, F .

GENE, 1978, 4 (02) :121-136

[5] A STANDARDIZED VECTOR SYSTEM FOR MANIPULATION AND ENHANCED EXPRESSION OF GENES IN ESCHERICHIA-COLI [J].

BOTTERMAN, J ;

ZABEAU, M .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1987, 6 (06) :583-591

[6] IMPROVED OLIGONUCLEOTIDE SITE-DIRECTED MUTAGENESIS USING M13 VECTORS [J].

CARTER, P ;

BEDOUELLE, H ;

WINTER, G .

NUCLEIC ACIDS RESEARCH, 1985, 13 (12) :4431-4443

[7] PEMBL - A NEW FAMILY OF SINGLE STRANDED PLASMIDS [J].

DENTE, L ;

CESARENI, G ;

CORTESE, R .

NUCLEIC ACIDS RESEARCH, 1983, 11 (06) :1645-1655

[8] FUNCTIONAL-ANALYSIS OF BACTERIOPHAGE-F1 INTERGENIC REGION [J].

DOTTO, GP ;

ENEA, V ;

ZINDER, ND .

VIROLOGY, 1981, 114 (02) :463-473

[9] INTERFERENCE RESISTANT MUTANTS OF PHAGE-F1 [J].

ENEA, V ;

ZINDER, ND .

VIROLOGY, 1982, 122 (01) :222-226

[10] OLIGONUCLEOTIDE-DIRECTED CONSTRUCTION OF MUTATIONS - A GAPPED DUPLEX DNA PROCEDURE WITHOUT ENZYMATIC-REACTIONS INVITRO [J].

FRITZ, HJ ;

HOHLMAIER, J ;

KRAMER, W ;

OHMAYER, A ;

WIPPLER, J .

NUCLEIC ACIDS RESEARCH, 1988, 16 (14) :6987-6999

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