RESPONSE-SURFACE OPTIMIZATION OF TEMPERATURE AND PH FOR THE GROWTH OF PACHYSOLEN-TANNOPHILUS

被引：18

作者：

ROEBUCK, K

BRUNDIN, A

JOHNS, M

机构：

[1] NORSK BIOFERM AS,SANDNES,NORWAY

[2] UNIV QUEENSLAND,DEPT CHEM ENGN,ST LUCIA,QLD 4067,AUSTRALIA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 01期

关键词：

OPTIMIZATION; CELL PRODUCTION; PACHYSOLEN TANNOPHILUS;

D O I：

10.1016/0141-0229(94)00046-T

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Response surface modeling was applied to shake-flask cultures of Pachysolen tannophilus, CBS 4044, to determine the optimum temperature and pH for growth. A two-factorial experiment was designed and the results were analyzed and plotted using MODDE software. This technique proved efficient at identifying the optimum temperature and pH by performing a limited number of fermentations while varying the two variables at the same time. The results indicated that the maximum yield of biomass density corresponded to pH 3.7 and a temperature of 31.5 degrees C, at an initial carbon source (D-xylose) concentration of approximately 50 g l(-1).

引用

页码：75 / 78

页数：4

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