RAPID SEPARATION OF BOVINE BETA-CRYSTALLIN SUBUNITS-BETA-B1, SUBUNIT-BETA-B2, SUBUNIT-BETA-B3, SUBUNIT-BETA-A3 AND SUBUNIT-BETA-A4

被引：65
作者：
SLINGSBY, C
BATEMAN, OA
机构：
[1] Birkbeck College, Laboratory of Molecular Biology, Department of Crystallography, London, WC1E 7HX, Malet Street
来源：
EXPERIMENTAL EYE RESEARCH | 1990年 / 51卷 / 01期
关键词：
eye lens proteins; Fast Protein Liquid Chromatography System; βH; βL1; βL2-crystallins;
D O I：
10.1016/0014-4835(90)90165-Q
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Bovine β-crystallin aggregates, βH-, βL1- and βL2-crystallins, prepared by rapid gel filtration, are each subjected to anion-exchange chromatography in deaggregating media using a Pharmacia Fast Protein Liquid Chromatography System. BetaB1, βB2 and βA4 subunits are rapidly isolated using a one step Mono Q column from βH-, βL2- and βL1-crystallin, respectively. BetaB3 and βA3 are separated from each other using a second Mono Q column starting from βL2- and βL1-crystallin respectively. Whereas βB2, βB3 and βA4 are common to all sizes of aggregate, βB1 is restricted to βH-crystallin and βA3 is absent from βL2-crystallin. © 1990.
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页码：21 / 26
页数：6
相关论文
共 19 条
[1]   IMPROVED RESOLUTION OF CALF LENS BETA-CRYSTALLINS [J].
ASSELBERGS, FAM ;
KOOPMANS, M ;
VANVENROOIJ, WJ ;
BLOEMENDAL, H .
EXPERIMENTAL EYE RESEARCH, 1979, 28 (02) :223-228
[2]   PROLINE-RICH AND ALANINE-RICH N-TERMINAL EXTENSION OF THE BASIC BOVINE BETA-CRYSTALLIN B1-CHAINS [J].
BERBERS, GAM ;
HOEKMAN, WA ;
BLOEMENDAL, H ;
DEJONG, WW ;
KLEINSCHMIDT, T ;
BRAUNITZER, G .
FEBS LETTERS, 1983, 161 (02) :225-229
[3]   HOMOLOGY BETWEEN THE PRIMARY STRUCTURES OF THE MAJOR BOVINE BETA-CRYSTALLIN CHAINS [J].
BERBERS, GAM ;
HOEKMAN, WA ;
BLOEMENDAL, H ;
DEJONG, WW ;
KLEINSCHMIDT, T ;
BRAUNITZER, G .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 139 (03) :467-479
[4]  
BERBERS GAM, 1982, EUR J BIOCHEM, V128, P495
[5]   STRUCTURAL ASPECTS OF BOVINE BETA-CRYSTALLINS - PHYSICAL CHARACTERIZATION INCLUDING DISSOCIATION-ASSOCIATION BEHAVIOR [J].
BINDELS, JG ;
KOPPERS, A ;
HOENDERS, HJ .
EXPERIMENTAL EYE RESEARCH, 1981, 33 (03) :333-343
[6]   RECOMMENDATIONS FOR CRYSTALLIN NOMENCLATURE [J].
BLOEMENDAL, H ;
PIATIGORSKY, J ;
SPECTOR, A .
EXPERIMENTAL EYE RESEARCH, 1989, 48 (04) :465-466
[7]  
de Jong W.W, 1981, MOL CELLULAR BIOL EY, P221, DOI DOI 10.1146/ANNUREV.ECOLSYS.33.020602.095451
[8]   PRIMARY STRUCTURE OF THE BOVINE BETA-CRYSTALLIN BP CHAIN - INTERNAL DUPLICATION AND HOMOLOGY WITH GAMMA-CRYSTALLIN [J].
DRIESSEN, HP ;
HERBRINK, P ;
BLOEMENDAL, H ;
DEJONG, WW .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 121 (01) :83-91
[9]   CLONING AND CHARACTERIZATION OF A COW BETA-CRYSTALLIN CDNA [J].
GORIN, MB ;
HORWITZ, J .
CURRENT EYE RESEARCH, 1984, 3 (07) :939-948
[10]  
Harding JJ, 1984, EYE, V16, P207
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