ACTINOBACILLUS-ACTINOMYCETEMCOMITANS MITOGENICITY FOR B-CELLS CAN BE ATTRIBUTED TO LIPOPOLYSACCHARIDE

被引：8
作者：
EASTCOTT, JW
TAUBMAN, MA
SMITH, DJ
HOLT, SC
机构：
[1] Department of Immunology, Forsyth Dental Center, Boston, Massachusetts
[2] Department of Periodontics, University of Texas Health Science Center, San Antonio, Texas
来源：
ORAL MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 5卷 / 01期
关键词：
Actinobacillus actinomycetemcomitans; athymic “nude” rats; B cells; lipopolysaccharide; mitogenicity; periodontal disease;
D O I：
10.1111/j.1399-302X.1990.tb00218.x
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
The purpose of this investigation was to determine the component(s) of whole Actinobacillus actinomycetemcomitans bacteria responsible for B cell mitogenic activity. Congenitally athymic “nude” rats were used as a source of B cells devoid of T lymphocyte activity. Spleen cells were cultured with, or without, whole formalin‐killed A. actinomycetemcomitans bacteria or with purified LPS from A. actinomycetemcomitans. Dose‐response curves to A. actinomycetemcomitans cells or to A. actinomycetemcomitans‐LPS showed that responses were dose dependent. If optimal quantities of both A. actinomycetemcomitans and A. actinomycetemcomitans‐LPS were added in combination, the proliferative responses were the same as if either was added alone, i.e., the responses were not additive. Polymyxin B at 2μg/well completely abrogated the proliferative response of athymic rat splenocytes to 107A. actinomycetemcomitans cells or to 1.25 μg A. actinomycetemcomitans‐LPS/well. Therefore, the in vitro early proliferative response of B cells to A. actinomycetemcomitans can be attributed to the presence of LPS on A. actinomycetemcomitans cells. A considerable portion of the in situ lymphocytic gingival response to A. actinomycetemcomitans infection seen in periodontal disease patients may be a B cell mitogenic response to the LPS of this bacterium. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：8 / 11
页数：4
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共 17 条
  • [1] Andersson J, Melchers F, Galanos C, Luderitz O., The mitogenic effect of lipopolysaccharide on bone marrow‐derived mouse lymphocytes, Lipid A as the mitogenic part of the molecule. J Exp Med, 137, pp. 943-953, (1973)
  • [2] Chiller JM, Skidmore BJ, Morrison DC, Weigle WO., Relationship of the structure of bacterial lipopolysaccharides to its function in mitogenesis and adjuvanticity, Proc Natl Acad Sci, 70, pp. 2129-2133, (1973)
  • [3] Hoover CI., Electrophoretic heterogeneity of lipopolysaccharides of Actinobacillus actinomycetemcomitans, J Dent Res, 67, pp. 574-576, (1988)
  • [4] Kiley P, Holt SC., Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27, Infect Immun, 30, pp. 862-873, (1980)
  • [5] Mallison SM, Szakal AK, Ranney RR, Tew JG., Antibody synthesis specific for nonoral antigens in inflamed gingiva, Infect Immun, 56, pp. 823-830, (1988)
  • [6] Nishihara T, Koga T, Hamada S., Extracellular proteinaceous substances from Haemophilus actinomycetemcomitans induce mitogenic responses in murine lymphocytes, Oral Microbiol Immunol, 2, pp. 48-52, (1987)
  • [7] Peavy DL, Shands JW, Adler WH, Smith RT., Mitogenicity of bacterial endotoxins: characterization of the mitogenic principle, J Immunol, 111, pp. 352-357, (1973)
  • [8] Shenker BJ, McArthur WP, Tsai C-C., Immune suppression induced by Actinobacillus actinomycetemcomitans. I. Effects on human peripheral blood lymphocyte responses to mitogens and antigens, J Immunol, 128, pp. 148-154, (1982)
  • [9] Spellman JM, Reed ND., Immune and mitogenic responses by BALB/c, C3H/HeJ, and nude mice to Brucella abortis bacterin and lipopolysaccharide, Infect Immun, 24, pp. 371-378, (1979)
  • [10] Taubman MA, Stoufi ED, Seymour GJ, Smith DJ, Ebersole JL., Immunoregulatory aspects of periodontal disease, Adv Dent Res, 2, pp. 328-333, (1988)
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