DEVELOPMENT OF SUITABLE LINKAGE FOR OLIGONUCLEOTIDE SYNTHESIS AND PRELIMINARY HYBRIDIZATION STUDIES ON OLIGONUCLEOTIDES SYNTHESIZED IN-SITU

被引：5
作者：
KUMAR, A
机构：
[1] National Institute Of Immunology, New Delhi-110067, Aruna Asaf Ali Marg
来源：
NUCLEOSIDES & NUCLEOTIDES | 1994年 / 13卷 / 10期
关键词：
D O I：
10.1080/15257779408013212
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The supports with disulphide and urethane linkage were prepared and their linkage stability was studied in ammonia solution. The urethane linkage was found to be more stable than the disulphide linkage. The simple hybridization with support linked oligonucleotide;de revealed the hybridization yields in the range of 10-15%. Interestingly, the triple helix hybridization yields were 92.4%,88.2%,72.5 %,86.4% and 78.7% for polystyrene urethane, CPG (500 degrees A) urethane, CPG (3000 degrees A) urethane, (500 degrees A) disulphide and CPG (3000 degrees A) disulphide respectively.
引用
收藏
页码：2125 / 2134
页数：10
相关论文
共 20 条
[1]   NOVEL METHOD TO MAP TRANSCRIPTS - EVIDENCE FOR HOMOLOGY BETWEEN AN ADENOVIRUS MESSENGER-RNA AND DISCRETE MULTIPLE REGIONS OF VIRAL GENOME [J].
DUNN, AR ;
HASSELL, JA .
CELL, 1977, 12 (01) :23-36
[2]   TISSUE SULFHYDRYL GROUPS [J].
ELLMAN, GL .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) :70-77
[3]  
Gait M. J, 1984, OLIGONUCLEOTIDE SYNT
[4]   COVALENT ATTACHMENT OF OLIGONUCLEOTIDES TO SOLID SUPPORTS [J].
GHOSH, SS ;
MUSSO, GF .
NUCLEIC ACIDS RESEARCH, 1987, 15 (13) :5353-5372
[5]   HYBRIDIZATION PROPERTIES OF IMMOBILIZED NUCLEIC-ACIDS [J].
GINGERAS, TR ;
KWOH, DY ;
DAVIS, GR .
NUCLEIC ACIDS RESEARCH, 1987, 15 (13) :5373-5390
[6]   COLONY HYBRIDIZATION - METHOD FOR ISOLATION OF CLONED DNAS THAT CONTAIN A SPECIFIC GENE [J].
GRUNSTEIN, M ;
HOGNESS, DS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (10) :3961-3965
[7]  
Khrapko K R, 1991, DNA Seq, V1, P375, DOI 10.3109/10425179109020793
[8]   IMMOBILIZATION OF DNA VIA OLIGONUCLEOTIDES CONTAINING AN ALDEHYDE OR CARBOXYLIC-ACID GROUP AT THE 5' TERMINUS [J].
KREMSKY, JN ;
WOOTERS, JL ;
DOUGHERTY, JP ;
MEYERS, RE ;
COLLINS, M ;
BROWN, EL .
NUCLEIC ACIDS RESEARCH, 1987, 15 (07) :2891-2909
[9]   A RAPID SOLID-PHASE METHOD FOR THE SYNTHESIS OF 3'-THIOL GROUP CONTAINING OLIGONUCLEOTIDES [J].
KUMAR, A .
NUCLEOSIDES & NUCLEOTIDES, 1993, 12 (07) :729-736
[10]  
KUMAR A, UNPUB NUCLEOSIDES NU
12