Determination Trace Levels of Vitamin C and Folic Acid in Urine Sample by Ultrasound-assisted Dispersive Liquid-Liquid Microextraction Method Coupled HPLC-UV

被引:9
作者
Akbari, Akbar [1 ]
Chamkouri, Narges [2 ]
Zadabdollah, Atefeh [2 ]
机构
[1] Abadan Sch Med Sci, Abadan, Iran
[2] Abadan Sch Med Sci, Student Res Comm, Abadan, Iran
关键词
Dispersive liquid-liquid microextraction; Folic acid; HPLC; Vitamin C; Ultrasound-assisted;
D O I
10.13005/ojc/320623
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new, rapid and highly sensitive and rapid method was proposed for the simultaneous determination trace levels of vitamin C and folic acid in the human urine samples. The inuuence affecting factors on the UA-DLLME such as type and volume of the dispersive and extraction solvent, pH, ionic strength and temperature of sample solution, sonication condition investigated and optimized. Under the optimized extractions, relative standard deviations (RSD) of the analyses less than 6% (n= 3) and detection limit of 1.65-2.05 ng mL(-1) respectively. USAEME - HPLC-UV was successfully applied for the simultaneous determination of trace levels of vitamin C and folic acid in human urine Samples.
引用
收藏
页码:3027 / 3033
页数:7
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Zheng Xiao-Hong, 2015, J Pharm Anal, V5, P269, DOI 10.1016/j.jpha.2015.05.004