Determination Trace Levels of Vitamin C and Folic Acid in Urine Sample by Ultrasound-assisted Dispersive Liquid-Liquid Microextraction Method Coupled HPLC-UV

被引：9

作者：

Akbari, Akbar ^{[1
]}

Chamkouri, Narges ^{[2
]}

Zadabdollah, Atefeh ^{[2
]}

机构：

[1] Abadan Sch Med Sci, Abadan, Iran

[2] Abadan Sch Med Sci, Student Res Comm, Abadan, Iran

来源：

ORIENTAL JOURNAL OF CHEMISTRY | 2016年 / 32卷 / 06期

关键词：

Dispersive liquid-liquid microextraction; Folic acid; HPLC; Vitamin C; Ultrasound-assisted;

D O I：

10.13005/ojc/320623

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, rapid and highly sensitive and rapid method was proposed for the simultaneous determination trace levels of vitamin C and folic acid in the human urine samples. The inuuence affecting factors on the UA-DLLME such as type and volume of the dispersive and extraction solvent, pH, ionic strength and temperature of sample solution, sonication condition investigated and optimized. Under the optimized extractions, relative standard deviations (RSD) of the analyses less than 6% (n= 3) and detection limit of 1.65-2.05 ng mL(-1) respectively. USAEME - HPLC-UV was successfully applied for the simultaneous determination of trace levels of vitamin C and folic acid in human urine Samples.

引用

页码：3027 / 3033

页数：7