GROUP-SPECIFIC 16S RIBOSOMAL-RNA HYBRIDIZATION PROBES TO DESCRIBE NATURAL COMMUNITIES OF METHANOGENS

被引:723
作者
RASKIN, L
STROMLEY, JM
RITTMANN, BE
STAHL, DA
机构
[1] NORTHWESTERN UNIV,INST TECHNOL,DEPT CIVIL ENGN,2145 SHERIDAN RD,EVANSTON,IL 60208
[2] UNIV ILLINOIS,DEPT MICROBIOL,URBANA,IL 61801
[3] UNIV ILLINOIS,DEPT VET PATHOBIOL,URBANA,IL 61801
[4] UNIV ILLINOIS,DEPT CIVIL ENGN,URBANA,IL 61801
关键词
D O I
10.1128/AEM.60.4.1232-1240.1994
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Eight oligonucleotides which are complementary to conserved tracts of 16S rRNA from phylogenetically defined groups of methanogens were designed and characterized for use as hybridization probes for studies in environmental and determinative microbiology. The target-group specificity and temperature of dissociation for each probe were characterized. In general, the probes were very specific for the target methanogens and did not hybridize to the rRNAs of nontarget methanogens. Together, the eight probes circumscribe methanogens now represented in pure culture (with the exception of members of the family Methanothermaceae). Three probes are order specific; two identify members of the order Methanobacteriales, and one is specific for the order Methanococcales. The fourth probe encompasses three families belonging to the order Methanomicrobiales, the third order within the current classification. The fifth probe is specific for the remaining family within this order (Methanosarcinaceae). Three additional probes encompass different genera within the Methanosarcinaceae.
引用
收藏
页码:1232 / 1240
页数:9
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