A TECHNIQUE FOR THE RAPID REMOVAL OF PROTEINS FROM CELL-EXTRACTS CONTAINING POLYSACCHARIDES

被引：4

作者：

BERI, RG

ROLLINGS, JE

机构：

[1] Biopolymer Laboratory, Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, 01609, MA

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 11期

关键词：

D O I：

10.1007/BF00159401

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The contaminating proteins in microbial cell extracts containing desired polysaccharides, can be rapidly precipitated by adjusting the pH of the solution. In the present research, 90% of the contaminating proteins in the alkali extract of Mucor rouxii were eliminated by lowering the pH, through addition of an organic acid. The acid for pH adjustment was chosen so that it formed a readymade buffer for use in subsequent chromatographic steps for recovery of the polysaccharide. The pH adjustment procedure thus offers a rapid and easily scaleable method for protein removal from cell extracts containing polysaccharides.

引用

页码：783 / 786

页数：4

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