A RECOMBINATION-BASED ASSAY DEMONSTRATES THAT THE FRAGILE-X SEQUENCE IS TRANSCRIBED WIDELY DURING DEVELOPMENT

被引：18
作者：
HANZLIK, AJ [1 ]
OSEMLAKHANZLIK, MM [1 ]
HAUSER, MA [1 ]
KURNIT, DM [1 ]
机构：
[1] UNIV MICHIGAN,SCH MED,HOWARD HUGHES MED INST,ANN ARBOR,MI 48109
来源：
NATURE GENETICS | 1993年 / 3卷 / 01期
关键词：
D O I：
10.1038/ng0193-44
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum.
引用
收藏
页码：44 / 48
页数：5
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