DIABETES AND GLUCOSE TRANSPORTER GENE-EXPRESSION IN RAT SMALL-INTESTINE

被引：70
作者：
MIYAMOTO, K
HASE, K
TAKETANI, Y
MINAMI, H
OKA, T
NAKABOU, Y
HAGIHIRA, H
机构：
[1] Department of Nutrition, School of Medicine, The University of Tokushima, Tokushima
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 181卷 / 03期
关键词：
D O I：
10.1016/0006-291X(91)92053-M
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The expressions of Na+-dependent glucose transporter (SGLT1) and five facilitative glucose transporter genes (GLUT1-5) in the small intestine of streptozotocin (STZ)-induced diabetic rats were examined by RNA blotting analysis. The transcripts of SGLT1 mRNA gave bands of 4.5 Kilobases(Kb) and 2.8Kb (very faint band). The levels of SGLT1 mRNA were significantly increased in 30- and 60-day STZ rats, but not changed in acute diabetic rats (2- to 10- day STZ rats). The GLUT2 mRNA levels changed in parallel with the D-galactose transport activity, being increased about 4-fold in 5-day STZ rats. The transcripts of GLUT5 mRNA gave three bands of 5.1Kb, 2.8Kb and 2.0Kb, whose levels were significantly reduced in 30- and 60-day STZ rats. These results suggest that the facilitative glucose transporter (GLUT2), in addition to the Na+-dependent glucose transporter (SGLT1), may play an important role in intestinal glucose transport in diabetic rats. © 1991.
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页码：1110 / 1117
页数：8
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