DISCRETE DOMAINS OF HUMAN U6 SNRNA REQUIRED FOR THE ASSEMBLY OF U4 U6 SNRNP AND SPLICING COMPLEXES

被引：42
|
作者：
BINDEREIF, A [1 ]
WOLFF, T [1 ]
GREEN, MR [1 ]
机构：
[1] HARVARD UNIV,DEPT BIOCHEM & MOLEC BIOL,CAMBRIDGE,MA 02138
来源：
EMBO JOURNAL | 1990年 / 9卷 / 01期
关键词：
snRNA; splicing; U4/U6; snRNP;
D O I：
10.1002/j.1460-2075.1990.tb08102.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.
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页码：251 / 255
页数：5
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