NONAMPLIFYING ALLELES AT MICROSATELLITE LOCI - A CAUTION FOR PARENTAGE AND POPULATION STUDIES

被引：569

作者：

PEMBERTON, JM ^{[1
]}

SLATE, J ^{[1
]}

BANCROFT, DR ^{[1
]}

BARRETT, JA ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT GENET,CAMBRIDGE CB2 3EH,ENGLAND

来源：

MOLECULAR ECOLOGY | 1995年 / 4卷 / 02期

关键词：

CERVUS ELAPHUS; MICROSATELLITE; NONAMPLIFYING ALLELES; PATERNITY; PCR; RED DEER;

D O I：

10.1111/j.1365-294X.1995.tb00214.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

While genotyping wild red deer (Cervus elaphus) at microsatellite loci for paternity assignment, we found three loci (MAF65, BOVIRBP and Ce1JP23) with segregating nonamplifying alleles. Nonamplifying alleles were detected through mismatches between known mother-off spring pairs and by significant deviations from Hardy-Weinberg equilibria. In a wide range of molecular ecology applications, and especially in parentage assignment, the possible existence of undetectable alleles must be taken into account; this may be particularly important for microsatellite data.

引用

页码：249 / 252

页数：4

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