ESTROGEN-RECEPTORS IN 699 PRIMARY BREAST CANCERS - A COMPARISON OF IMMUNOHISTOCHEMICAL AND BIOCHEMICAL METHODS

被引：29

作者：

MOLINO, A

MICCIOLO, R

TURAZZA, M

BONETTI, F

PIUBELLO, Q

CORGNATI, A

SPEROTTO, L

MARTIGNONI, G

BONETTI, A

NORTILLI, R

CASTELLI, P

RODELLA, S

CAPELLI, P

MANFRIN, E

PELOSI, G

CETTO, GL

机构：

[1] UNIV VERONA,DEPT MED ONCOL,I-37100 VERONA,ITALY

[2] UNIV VERONA,DEPT PATHOL,I-37100 VERONA,ITALY

[3] UNIV VERONA,DEPT LAB,I-37100 VERONA,ITALY

[4] CIV HOSP,DEPT PATHOL,VERONA,ITALY

[5] CIV HOSP,NEGRAR,ITALY

[6] UNIV TRENT,INST STAT,TRENT,ITALY

来源：

BREAST CANCER RESEARCH AND TREATMENT | 1995年 / 34卷 / 03期

关键词：

BIOCHEMISTRY; BREAST CANCER; ESTROGEN RECEPTORS; IMMUNOHISTOCHEMISTRY;

D O I：

10.1007/BF00689713

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Over the last few years, estrogen receptor (ER) determination by immunohistochemistry (ERICA) has been extensively used, but it still remains to be established whether this method can replace the standard biochemical technique using dextran-coated charcoal (ERDCC). Patients and methods: ER were determined by both the dextran-coated charcoal (DCC) method and immunohistochemistry (ICA) in 699 patients with primary breast cancer; other parameters (age, pathological T-pT-and nodal status-pN-, progesterone receptors by DCC, proliferative index by ICA) were also recorded. The 'best' cut-off for ERICA was evaluated by means of Receiver Operating Characteristics (R.O.C.) analysis; logistic regression analysis was used to find adequate 'weights' for stain intensity. Results and conclusions: A significant correlation was found between the two methods (p < 0.001). R.O.C. analysis revealed that the 'best' cut-off for the ERICA score was 45% (sensitivity 0.810, specificity 0.804). Logistic regression analysis showed that an ERICA score which also considers staining intensity does not add any useful information concerning ER content in breast cancers.

引用

页码：221 / 228

页数：8

共 36 条

[21] Pertschuk L.P., Kim D.S., Nayer K., Feldman J.G., Eisenberg K.B., Carter A.C., Rong Z.T., Thelmo W.L., Fleisher J., Greene G.L., Immunocytochemical estrogen and progestin receptor assays in breast cancer with monoclonal antibodies, Cancer, 66, pp. 1663-1670, (1990)
[22] Katz R.L., Patel S., Sneige N., Fritsche H.A., Hortobagyi G.N., Ames F.C., Brooks T., Ordonez N.G., Comparison of immunocytochemical and biochemical assays for estrogen receptor in fine needle aspirates and histologic sections from breast carcinomas, Breast Cancer Res Treat, 15, pp. 191-203, (1990)
[23] Allred D.C., Bustamante M.A., Daniel C.O., Gaskill H.V., Cruz A.B., Immunocytochemical analysis of estrogen receptors in human breast carcinomas, Arch Surg, 125, pp. 107-113, (1990)
[24] TNM Atlas. Illustrated guide to the TNM/pTNM classification of malignant tumours, (1992)
[25] Revision of standards for the assessment of hormone receptors in human breast cancer: report of the second EORTC workshop, held on March 16–17, 1979, in the Netherlands Cancer Institute, Eur J Cancer, 16, pp. 1513-1515, (1980)
[26] Gerdes J., Pickartz H., Brotherton J., Hammerstein J., Weitzel H., Stein H., Growth fractions and estrogen receptors in human breast cancers as determined in situ with monoclonal antibodies, Am J Pathol, 129, pp. 486-492, (1987)
[27] Metz C.E., Basic principles of R.O.C. analysis, Semin Nucl Med, 8, pp. 238-298, (1978)
[28] McNeil B.J., Adelstein S.P., Determining the value of diagnostic and screening tests, J Nucl Med, 17, pp. 439-448, (1976)
[29] Weinstein J., Finenberg R., Clinical Decision Analysis, pp. 114-127, (1980)
[30] Cudahy T.J., Boeryd B.R., Franlund B.K., Nordenskjold B.A., A comparison of three different methods for the determination of estrogen receptors in human breast cancer, Am J Clin Pathol, 90, pp. 583-590, (1988)

← 1 2 3 4 →