ESTROGEN-RECEPTORS IN 699 PRIMARY BREAST CANCERS - A COMPARISON OF IMMUNOHISTOCHEMICAL AND BIOCHEMICAL METHODS

被引：29
作者：
MOLINO, A
MICCIOLO, R
TURAZZA, M
BONETTI, F
PIUBELLO, Q
CORGNATI, A
SPEROTTO, L
MARTIGNONI, G
BONETTI, A
NORTILLI, R
CASTELLI, P
RODELLA, S
CAPELLI, P
MANFRIN, E
PELOSI, G
CETTO, GL
机构：
[1] UNIV VERONA,DEPT MED ONCOL,I-37100 VERONA,ITALY
[2] UNIV VERONA,DEPT PATHOL,I-37100 VERONA,ITALY
[3] UNIV VERONA,DEPT LAB,I-37100 VERONA,ITALY
[4] CIV HOSP,DEPT PATHOL,VERONA,ITALY
[5] CIV HOSP,NEGRAR,ITALY
[6] UNIV TRENT,INST STAT,TRENT,ITALY
来源：
BREAST CANCER RESEARCH AND TREATMENT | 1995年 / 34卷 / 03期
关键词：
BIOCHEMISTRY; BREAST CANCER; ESTROGEN RECEPTORS; IMMUNOHISTOCHEMISTRY;
D O I：
10.1007/BF00689713
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: Over the last few years, estrogen receptor (ER) determination by immunohistochemistry (ERICA) has been extensively used, but it still remains to be established whether this method can replace the standard biochemical technique using dextran-coated charcoal (ERDCC). Patients and methods: ER were determined by both the dextran-coated charcoal (DCC) method and immunohistochemistry (ICA) in 699 patients with primary breast cancer; other parameters (age, pathological T-pT-and nodal status-pN-, progesterone receptors by DCC, proliferative index by ICA) were also recorded. The 'best' cut-off for ERICA was evaluated by means of Receiver Operating Characteristics (R.O.C.) analysis; logistic regression analysis was used to find adequate 'weights' for stain intensity. Results and conclusions: A significant correlation was found between the two methods (p < 0.001). R.O.C. analysis revealed that the 'best' cut-off for the ERICA score was 45% (sensitivity 0.810, specificity 0.804). Logistic regression analysis showed that an ERICA score which also considers staining intensity does not add any useful information concerning ER content in breast cancers.
引用
收藏
页码：221 / 228
页数：8
相关论文
共 36 条
  • [1] Wollenweider-Zerargui L., Barrelet L., Wong Y., Lemarchand-Beraud I., Gomez F., The predictive value of estrogen and progesterone receptor concentrations on the clinical behavior of breast cancer in women. Clinical correlations in 547 patients, Cancer, 57, pp. 1171-1180, (1988)
  • [2] Willia, Todd J.H., Ellis I.O., Dowle C.S., Haybittle J.L., Elston C.W., Nicholson R.I., Griffiths K., Blamey R.W., Oestrogen receptors in primary and advanced breast cancer. An eight-year review of 704 cases, Br J Cancer, 55, pp. 67-73, (1987)
  • [3] Alexieva-Figusch J., van Putten W.L., Blankestein M., The prognostic value and relationships of patient characteristics, estrogen and progestin receptors, and site of relapse in primary breast cancer, Cancer, 61, pp. 758-768, (1988)
  • [4] Clark G.M., McGuire W.L., Steroid receptors and other prognostic factors in primary breast cancer, Semin Oncol, 15, pp. 20-25, (1988)
  • [5] Molino A., Turazza M., Bonetti A., Biondani P., Griso C., Adami L., Corgnati A., Nortilli R., Pollini G.P., Modena S., Cetto G.L., Estrogen and progesterone receptors in breast cancer: correlation with clinical and pathological features and with prognosis, Oncology, 49, pp. 82-88, (1992)
  • [6] Systemic treatment of early breast cancer by hormonal, cytotoxic or immune therapy, Lancet, 339, pp. 1-15, (1992)
  • [7] Pertschuk L.P., Eisenberg K.B., Carter A.C., Feldman J., Immunohistologic localization of estrogen receptors in breast cancer with monoclonal antibodies, Cancer, 55, pp. 1513-1518, (1985)
  • [8] McCarty K.S., Miller L.S., Cox E.B., Konrath J., McCarty K.S., Estrogen receptor analysis. Correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies, Arch Path Lab Med, 109, pp. 716-721, (1985)
  • [9] Pascal R.R., Santeusano G., Sarrel D., Johnson C.E., Immunohistologic detection of estrogen receptors in paraffin-embedded breast cancers: correlation with cytosol measurements, Hum Path, 17, pp. 370-375, (1986)
  • [10] Reiner A., Reiner G., Spona J., Teleky B., Holzner J.H., Estrogen receptor analysis and biopsies and fine-needle aspirates from human breast carcinoma: correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies, Am J Pathol, 125, pp. 443-449, (1986)
1234