PRODUCTION AND GENETIC-CHARACTERIZATION OF NEAR-ISOGENIC LINES IN THE BREAD-WHEAT CULTIVAR ALPE

被引：22

作者：

POGNA, NE ^{[1
]}

REDAELLI, R ^{[1
]}

VACCINO, P ^{[1
]}

BIANCARDI, AM ^{[1
]}

PERUFFO, ADB ^{[1
]}

CURIONI, A ^{[1
]}

METAKOVSKY, EV ^{[1
]}

PAGLIARICCI, S ^{[1
]}

机构：

[1] UNIV PADUA,DIPARTIMENTO BIOTECNOL AGR,PADUA,ITALY

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 05期

关键词：

NEAR-ISOGENIC LINES; TRITICUM AESTIVUM; GLUTENIN; GLIADIN; MUTATION; BIOTYPES;

D O I：

10.1007/BF00222129

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Two biotypes of the bread-wheat cultivar Alpe were shown to possess contrasting alleles at each of the glutenin (Glu-B1, Glu-D1, Glu-B3 and Glu-D3) and gliadin (Gli-B1 and Gli-D1) loci on chromosomes 1B and 1D. Fourteen near-isogenic lines (NILs) were produced by crossing these biotypes and used to determine the genetic control of both low-molecular-weight (LMW) glutenin subunits and gliadins by means of one-dimensional or two-dimensional electrophoresis. Genes coding for the B, C and D groups of LMW subunits were found to be inherited in clusters tightly linked with those controlling gliadins. Southern-blot analysis of total genomic DNAs hybridized to a gamma-gliadin-specific cDNA clone revealed that seven NILs lack both the Gli-D1 and Glu-D3 loci on chromosome 1D. Segregation data indicated that these ''null'' alleles are normally inherited. Comparison of the ''null'' NILs with those possessing allele b at the Glu-D3 locus showed one B subunit, seven C subunits and two D subunits, as fractionated by two-dimensional A-PAGExSDS-PAGE, to be encoded by this allele. Alleles b and k at Glu-B3 were found to code for two C subunits plus eight and six B subunits respectively, whereas alleles b and k at Gli-B1 each controlled the synthesis of two beta-gliadins, one gamma- and two omega-gliadins. The novel Gli-BS locus coding for two omega-gliadins was shown to recombine with the Gli-B1 locus on chromosome 1B. The two-dimensional map of glutenin subunits showed alpha-gliadins encoded at the Gli-A2 locus on chromosome 6A. The use of Alpe NILs in the study of the individual and combined effects of glutenin subunits on dough properties is discussed.

引用

页码：650 / 658

页数：9

共 49 条

[1]

ALLARD R. W., 1956, HILGARDIA, V24, P235

[2]

AVIVI L, 1978, 5 INT WHEAT GEN S NE, P372

[3] MOLECULAR ANALYSIS OF GAMMA-GLIADIN GENE FAMILIES AT THE COMPLEX GLI-1 LOCUS OF BREAD WHEAT (TRITICUM-AESTIVUM L) [J].