SOLID-PHASE SYNTHESIS OF 5'-DIPHOSPHORYLATED OLIGORIBONUCLEOTIDES AND THEIR CONVERSION TO CAPPED M(7)GPPP-OLIGORIBONUCLEOTIDES FOR USE AS PRIMERS FOR INFLUENZA-A VIRUS-RNA POLYMERASE IN-VITRO

被引：25

作者：

BROWNLEE, GG ^{[1
]}

FODOR, E ^{[1
]}

PRITLOVE, DC ^{[1
]}

GOULD, KG ^{[1
]}

DALLUGE, JJ ^{[1
]}

机构：

[1] UNIV UTAH,DEPT BIOCHEM,SALT LAKE CITY,UT 84132

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 14期

关键词：

D O I：

10.1093/nar/23.14.2641

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have synthesized four different 5'-diphosphorylated oligoribonucleotides, varying in length from 11 to 13 nucleotides by a new solid phase method. After deprotection and partial purification the 5'-diphosphorylated oligoribonucleotides could be converted to capped (m(7)Gppp)-oligoribonucleotides using guanylyl transferase. Radiolabelled capped oligoribonucleotides acted as primers for the influenza A virus RNA polymerase in vitro. The solid phase method described here should also allow the addition of 5'-diphosphates to synthetic oligodeoxyribonucleotides and be capable of automation.

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页码：2641 / 2647

页数：7

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