MEMBRANE AFFINITY-CHROMATOGRAPHY OF ALKALINE-PHOSPHATASE

被引：45

作者：

GUO, W ^{[1
]}

SHANG, ZH ^{[1
]}

YU, YN ^{[1
]}

ZHOU, LM ^{[1
]}

机构：

[1] ACAD SINICA,DALIAN INST CHEM PHYS,DALIAN 116012,PEOPLES R CHINA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 685卷 / 02期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/0021-9673(94)00725-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the preparation of a microporous cellulose membrane is described. The triazine dyes Cibacron Blue F3GA and Active Red K2BP were immobilized as affinity ligands. Up to 90 mg of Active Red K2BP can be coupled to 1 g of membrane matrix. A membrane cartridge containing red affinity membranes was also prepared. The flux of the cartridge containing 80 sheets of membrane can reach 5.0 ml/min with a pressure drop of 0.1 MPa. On this cartridge, the chromatography of alkaline phosphatase was performed with a 60% recovery of activity and a 40-fold purification.

引用

页码：344 / 348

页数：5

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