COMPARISON OF GEN-PROBE GROUP-A STREPTOCOCCUS DIRECT TEST WITH CULTURE FOR DIAGNOSING STREPTOCOCCAL PHARYNGITIS

被引:29
作者
POKORSKI, SJ
VETTER, EA
WOLLAN, PC
COCKERILL, FR
机构
[1] MAYO CLIN & MAYO FDN,DEPT LAB MED,DIV CLIN MICROBIOL,ROCHESTER,MN 55905
[2] MAYO CLIN & MAYO FDN,DEPT INTERNAL MED,DIV INFECT DIS,ROCHESTER,MN 55905
[3] MAYO CLIN & MAYO FDN,DEPT HLTH SCI RES,BIOSTAT SECT,ROCHESTER,MN 55905
关键词
D O I
10.1128/JCM.32.6.1440-1443.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Group A Streptococcus Direct Test (GP-ST test; Gen-Probe, Inc., San Diego, Calif.) was compared with culture for the detection of Streptococcus pyogenes from throat swabs of 767 patients with pharyngitis. Swabs were tested by the GP-ST test after inoculating a 5% sheep blood agar (SBA) plate. SBA plates were incubated at 35 degrees C in room air for 72 h. SBA plates with no evidence of beta-hemolytic colonies after 18 to 24 h of incubation were subcultured by taking a swipe across the primary inoculum from the SBA plate to an agar selective for Streptococcus spp. In a low-prevalence (11.9%) population and in comparison with the number of positive cultures detected by the 72-h single-culture method (SBA plate method), the GP-ST test had a sensitivity of 88.6%, a specificity of 97.8%, a positive predictive value of 83.9%, and a negative predictive value of 98.5%. In comparison with the growth of any colonies of S. pyogenes on the 72-h SBA plates plus a subculture onto selective blood agar, the sensitivities and specificities were as follows: 72-h SBA plate method, 96.7 and 100%, respectively; GP-ST test, 85.7 and 97.8%, respectively. The GP-ST test is an easy-to-perform, reliable test for batch screening of throat swabs for S. pyogenes.
引用
收藏
页码:1440 / 1443
页数:4
相关论文
共 7 条
[1]   COMPARISON OF 3 METHODS FOR DETECTION OF GROUP-A STREPTOCOCCI IN THROAT SWABS [J].
ANHALT, JP ;
HEITER, BJ ;
NAUMOVITZ, DW ;
BOURBEAU, PP .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (08) :2135-2138
[2]   GROUP-A STREPTOCOCCAL INFECTIONS AND ACUTE RHEUMATIC-FEVER [J].
BISNO, AL .
NEW ENGLAND JOURNAL OF MEDICINE, 1991, 325 (11) :783-793
[3]   NOVEL, RAPID OPTICAL IMMUNOASSAY TECHNIQUE FOR DETECTION OF GROUP-A STREPTOCOCCI FROM PHARYNGEAL SPECIMENS - COMPARISON WITH STANDARD CULTURE METHODS [J].
HARBECK, RJ ;
TEAGUE, J ;
CROSSEN, GR ;
MAUL, DM ;
CHILDERS, PL .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (04) :839-844
[4]  
HEITER BJ, 1992, J CLIN MICROBIOL, V31, P2070
[5]   EFFECTS OF TRIMETHOPRIM-SULFAMETHOXAZOLE AND INCUBATION ATMOSPHERE ON ISOLATION OF GROUP-A STREPTOCOCCI [J].
LIBERTIN, CR ;
WOLD, AD ;
WASHINGTON, JA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1983, 18 (03) :680-682
[6]   RAPID DETECTION OF STREPTOCOCCUS-PYOGENES IN PEDIATRIC-PATIENT SPECIMENS BY DNA-PROBE [J].
STEED, LL ;
KORGENSKI, EK ;
DALY, JA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (11) :2996-3000
[7]   INSENSITIVITY OF RAPID ANTIGEN-DETECTION METHODS AND SINGLE BLOOD AGAR PLATE CULTURE FOR DIAGNOSING STREPTOCOCCAL PHARYNGITIS [J].
WEGNER, DL ;
WITTE, DL ;
SCHRANTZ, RD .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1992, 267 (05) :695-697