DEVELOPMENT OF A HOST VECTOR SYSTEM IN A RHODOCOCCUS STRAIN AND ITS USE FOR EXPRESSION OF THE CLONED NITRILE HYDRATASE GENE-CLUSTER

被引：80
作者：
HASHIMOTO, Y
NISHIYAMA, M
YU, F
WATANABE, I
HORINOUCHI, S
BEPPU, T
机构：
[1] UNIV TOKYO,DEPT AGR CHEM,YAYOI 1-1-1,BUNKYO KU,TOKYO 113,JAPAN
[2] NITTO CHEM IND CO LTD,TSURUMI KU,YOKOHAMA 230,JAPAN
来源：
JOURNAL OF GENERAL MICROBIOLOGY | 1992年 / 138卷
关键词：
D O I：
10.1099/00221287-138-5-1003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Two different types of plasmid were isolated from strains of Rhodococcus rhodochrous. Two plasmids, of the same type but from different strains, were combined with Escherichia coli plasmids carrying antibiotic resistance markers to develop E. coli-Rhodococcus shuttle vectors. The ampicillin and kanamycin resistance markers served for selection in Rhodococcus. Electroporation was used to introduce recombinant plasmid DNA into R. rhodochrous ATCC 12674 at a frequency of 5 x 10(7) transformants per pg DNA. With these host-vector and transformation systems, the nitrile hydratase and amidase genes of a Rhodococcus strain were introduced into the host strain and were efficiently expressed.
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页码：1003 / 1010
页数：8
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