CORE ANTENNA COMPLEXES, CP43 AND CP47, OF HIGHER-PLANT PHOTOSYSTEM-II - SPECTRAL PROPERTIES, PIGMENT STOICHIOMETRY, AND AMINO-ACID-COMPOSITION

被引:106
作者
ALFONSO, M
MONTOYA, G
CASES, R
RODRIGUEZ, R
PICOREL, R
机构
[1] CSIC,ESTAC EXPTL AULA DEI,DEPT NUTR VEGETAL,E-50080 ZARAGOZA,SPAIN
[2] UNIV ZARAGOZA,CSIC,INST CIENCIA MAT ARAGON,E-50090 ZARAGOZA,SPAIN
[3] UNIV COMPLUTENSE MADRID,FAC CIENCIAS QUIM,DEPT BIOQUIM & BIOL MOLEC,E-28040 MADRID,SPAIN
关键词
D O I
10.1021/bi00200a034
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The core antenna complexes of photosystem II, CP43 and CP47, were purified from two higher plants by anion-exchange chromatography, using a combination of the chaotropic agent LiClO4 and the nonionic detergent beta-dodecyl maltoside. The Q(y) transition was resolved at 48 K into two main bands near 682.3 and 671.5 nm for CP43, while the CP47 spectrum showed a more complex structure with main bands at 688, 681.2, 676, 670, 667, and 661 nm. Emission bands (77 K) were detected at 683 and 695 nm for CP43 and CP47, respectively. Fluorescence excitation spectra showed high efficiency of energy transfer between the different transitions of the chlorophylls and a somewhat lower efficiency from beta-carotene. The circular dichroism spectrum of CP47 indicated the presence of excitonic interactions between some chlorophylls. In contrast, CP43 showed a single negative circular dichroism band at 670 nm. The pigment content of the complexes was determined by both spectroscopic measurements and HPLC. Contents of 18 chlorophylls a and 5 beta-carotenes per CP43 polypeptide and 19 chlorophylls a and 3 beta-carotenes per CP47 polypeptide were found, using the methods of Lowry or Bradford for protein quantitation. When the protein concentration was determined from the amino acid analysis, 20 chlorophylls a and 5 beta-carotenes per CP43 and 21-22 chlorophylls a and 4 beta-carotenes per CP47 were obtained. Thus, a content of 46-48 chlorophylls a was obtained for the core complex, assuming 4-6 chlorophylls per reaction center, in agreement with the composition obtained experimentally using a highly purified oxygen-evolving core complex. This suggested that no pigments were lost during the purification procedure. Moreover, the amino acid analysis of the purified complexes revealed a high homology with the amino acid composition derived from the gene sequences reported for other higher plants.
引用
收藏
页码:10494 / 10500
页数:7
相关论文
共 38 条
[1]   DISINTEGRATION AND RECONSTITUTION OF PHOTOSYSTEM-II REACTION CENTER CORE COMPLEX .1. PREPARATION AND CHARACTERIZATION OF 3 DIFFERENT TYPES OF SUBCOMPLEX [J].
AKABORI, K ;
TSUKAMOTO, H ;
TSUKIHARA, J ;
NAGATSUKA, T ;
MOTOKAWA, O ;
TOYOSHIMA, Y .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 932 (03) :345-357
[2]  
BARBATO R, 1991, FEBS LETT, V286, P286
[3]   ULTRAVIOLET ABSORPTION SPECTRA OF PROTEINS AND AMINO ACIDS [J].
BEAVEN, GH ;
HOLIDAY, ER .
ADVANCES IN PROTEIN CHEMISTRY, 1952, 7 :319-386
[4]   A HIGHLY RESOLVED, OXYGEN-EVOLVING PHOTOSYSTEM-II PREPARATION FROM SPINACH THYLAKOID MEMBRANES - ELECTRON-PARAMAGNETIC-RES AND ELECTRON-TRANSPORT PROPERTIES [J].
BERTHOLD, DA ;
BABCOCK, GT ;
YOCUM, CF .
FEBS LETTERS, 1981, 134 (02) :231-234
[5]  
BOWLBY NR, 1988, LIGHT ENERGY TRANSDU, P215
[6]   D1-D2-CYTOCHROME B559 COMPLEX FROM THE AQUATIC PLANT SPIRODELA-OLIGORRHIZA - CORRELATION BETWEEN COMPLEX INTEGRITY, SPECTROSCOPIC PROPERTIES, PHOTOCHEMICAL ACTIVITY, AND PIGMENT COMPOSITION [J].
BRAUN, P ;
GREENBERG, BM ;
SCHERZ, A .
BIOCHEMISTRY, 1990, 29 (45) :10376-10387
[7]   CLOSE ASSOCIATION OF THE 33-KDA EXTRINSIC PROTEIN WITH THE APOPROTEIN OF CPA1 IN PHOTOSYSTEM-II [J].
BRICKER, TM ;
ODOM, WR ;
QUEIROLO, CB .
FEBS LETTERS, 1988, 231 (01) :111-117
[8]   THE STRUCTURE AND FUNCTION OF CPA-1 AND CPA-2 IN PHOTOSYSTEM-II [J].
BRICKER, TM .
PHOTOSYNTHESIS RESEARCH, 1990, 24 (01) :1-13
[10]  
Dawson R.M.C., 1986, DATA BIOCH RES