HEXAMETHYLDISILAZANE AS A DRYING AGENT FOR POLLEN SCANNING ELECTRON-MICROSCOPY

被引：50
作者：
CHISSOE, WF
VEZEY, EL
SKVARLA, JJ
机构：
[1] UNIV OKLAHOMA, SAMUEL ROBERTS NOBLE ELECTRON MICROSCOPY LAB, NORMAN, OK 73019 USA
[2] OKLAHOMA BIOL SURVEY, NORMAN, OK 73019 USA
来源：
BIOTECHNIC & HISTOCHEMISTRY | 1994年 / 69卷 / 04期
关键词：
HEXAMETHYLDISILAZANE; HMDS; POLLEN; SEM;
D O I：
10.3109/10520299409106286
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Use of hexamethyldisilazane (HMDS) as a final dehydrating solution provides robust, undistorted secondary electron images of a variety of angiosperm and gymnosperm pollen grains, including those considered to be susceptible to collapse in the scanning electron microscope. Ease of handling, low cost, lack of specialized equipment, minimal expenditure of time, and high rate of success are factors that favor HMDS over other drying agents for preparing pollen grains for scanning electron microscopy.
引用
收藏
页码：192 / 198
页数：7
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