SCREENING OF TRANSGENIC PLANTS WITH POLYMERASE CHAIN-REACTION

被引:0
作者
PADEGIMAS, L
SHULGA, OA
SKRYABIN, KG
机构
关键词
POLYMERASE CHAIN REACTION; TRANSGENIC PLANTS; PLANT SCREENING;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A test is system is described for screening transgenic plants using polymerase chain reaction (PCR). The technique requires no optimization of PCR conditions for every transferred gene, and is applicable to screening of regenerants after transformation with Agrobacterium carrying the pBIN19 vector or its derivative. In addition, a simple procedure is offered for preparing small samples of plant genomic DNA suitable for the PCR assay.
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页码:583 / 585
页数:3
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