3 TYPES OF SODIUM-CHANNELS IN ADULT-RAT DORSAL-ROOT GANGLION NEURONS

被引:264
作者
CAFFREY, JM
ENG, DL
BLACK, JA
WAXMAN, SG
KOCSIS, JD
机构
[1] YALE UNIV, SCH MED, DEPT NEUROL, LCI 707, 333 CEDAR ST, NEW HAVEN, CT 06510 USA
[2] DEPT VET AFFAIRS MED CTR, NEUROSCI RES CTR, West Haven, CT 06516 USA
关键词
SODIUM CHANNEL; DORSAL ROOT GANGLION; TTX; VOLTAGE CLAMP;
D O I
10.1016/0006-8993(92)91687-A
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Several types of Na+ currents have previously been demonstrated in dorsal root ganglion (DRG) neurons isolated from neonatal rats, but their expression in adult neurons has not been studied. Na+ current properties in adult dorsal root ganglion (DRG) neurons of defined size class were investigated in isolated neurons maintained in primary culture using a combination of microelectrode current clamp, patch voltage clamp and immunocytochemical techniques. Intracellular current clamp recordings identified differing relative contributions of TTX-sensitive and -resistant inward currents to action potential waveforms in DRG neuronal populations of defined size. Patch voltage clamp recordings identified three distinct kinetic types of Na+ current differentially distributed among these size classes of DRG neurons. 'Small' DRG neurons co-express two types of Na+ current: (i) a rapidly-inactivating, TTX-sensitive 'fast' current and (ii) a slowly-activating and -inactivating, TTX-resistant 'slow' current. The TTX-sensitive Na+ current in these cells was almost completely inactivated at typical resting potentials. 'Large' cells expressed a single TTX-sensitive Na+ current identified as 'intermediate' by its inactivation rate constants. 'Medium'-sized neurons either co-expressed 'fast' and 'slow' current or expressed only 'intermediate' current. Na+ channel expression in these size classes was also measured by immunocytochemical techniques. An antibody against brain-type Na+ channels (Ab7493)10 labeled small and large neurons with similar intensity. These results demonstrate that three types of Na+ currents can be detected which correlate with electrogenic properties of physiologically and anatomically distinct populations of adult rat DRG neurons.
引用
收藏
页码:283 / 297
页数:15
相关论文
共 41 条
[1]  
ALDRICH RW, 1987, J NEUROSCI, V7, P418
[2]   A REINTERPRETATION OF MAMMALIAN SODIUM-CHANNEL GATING BASED ON SINGLE CHANNEL RECORDING [J].
ALDRICH, RW ;
COREY, DP ;
STEVENS, CF .
NATURE, 1983, 306 (5942) :436-441
[3]   GLIAL AND NEURONAL FORMS OF THE VOLTAGE-DEPENDENT SODIUM-CHANNEL - CHARACTERISTICS AND CELL-TYPE DISTRIBUTION [J].
BARRES, BA ;
CHUN, LLY ;
COREY, DP .
NEURON, 1989, 2 (04) :1375-1388
[4]   ELECTRON-MICROSCOPIC SERIAL SECTION ANALYSIS OF NODES OF RANVIER IN LUMBOSACRAL SPINAL ROOTS OF THE CAT - ULTRASTRUCTURAL ORGANIZATION OF NODAL COMPARTMENTS IN FIBERS OF DIFFERENT SIZES [J].
BERTHOLD, CH ;
RYDMARK, M .
JOURNAL OF NEUROCYTOLOGY, 1983, 12 (03) :475-505
[5]  
BLACK JA, 1989, P ROY SOC LOND B BIO, V238, P38
[6]   SODIUM AND POTASSIUM CURRENTS IN ACUTELY DEMYELINATED INTERNODES OF RABBIT SCIATIC-NERVES [J].
CHIU, SY ;
SCHWARZ, W .
JOURNAL OF PHYSIOLOGY-LONDON, 1987, 391 :631-649
[7]  
DODD J, 1986, J EXP BIOL, V124, P225
[8]  
ECKERT R, 1986, PROGR BIOPHYS
[9]   RETRACTED: THE VOLTAGE-DEPENDENT SODIUM-CHANNEL IN MAMMALIAN CNS AND PNS - ANTIBODY CHARACTERIZATION AND IMMUNOCYTOCHEMICAL LOCALIZATION (RETRACTED ARTICLE. SEE VOL 761, PG 2, 1997) [J].
ELMER, LW ;
BLACK, JA ;
WAXMAN, SG ;
ANGELIDES, KJ .
BRAIN RESEARCH, 1990, 532 (1-2) :222-231
[10]  
ELMER LW, 1988, THESIS BAYLOR COLLEG