Ornamental Plant Transformation

被引:5
作者
Brand, Mark H. [1 ]
机构
[1] Univ Connecticut, Dept Plant Sci, 1376 Storrs Rd, Storrs, CT 06269 USA
关键词
Agrobacterium-mediated transformation; microprojectile bombardment; transgenic; ornamental; gene gun; biolistics;
D O I
10.1300/J411v17n01_02
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Ornamental plant transformation has advanced considerably in the last decade. Now over 40 genera have been reported to be transformed. The primary methods of creating transgenic ornamental species have been Agrobacterium tumefaciens-mediated transformation and microprojectile bombardment. The vast majority of reports indicate the use of Agrobacterium-mediated transformation employing binary vectors and vir helper plasmids or supervirulence genes. Many reports are of transformation with the uidA reporter gene driven by the 35S cauliflower mosaic virus promoter, but recent efforts are now focusing on trait genes including disease resistance, flower color, flower longevity, floral scent and plant habit. Greater use of tissue specific and inducible promoters promises to enhance the functionality and usefulness of introduced trait genes. While technical challenges for production of transgenic ornamental plants still exist, the greatest challenges to realizing the potential benefits of transgenic ornamental plants are questionable public acceptance of transgenic plants and the prohibitive costs of generating environmental impact data needed to gain regulatory clearance. (C) 2006 by The Haworth Press, Inc. All rights reserved.
引用
收藏
页码:27 / 50
页数:24
相关论文
共 103 条
[1]   Modification of flower color in torenia (Torenia fournieri Lind.) by genetic transformation [J].
Aida, R ;
Kishimoto, S ;
Tanaka, Y ;
Shibata, M .
PLANT SCIENCE, 2000, 153 (01) :33-42
[2]   Agrobacterium tumefaciens-mediated transformation of Cyclamen persicum Mill. [J].
Aida, R ;
Hirose, Y ;
Kishimoto, S ;
Shibata, M .
PLANT SCIENCE, 1999, 148 (01) :1-7
[3]   Transformation of the monocotyledonous Alstroemeria by Agrobacterium tumefaciens [J].
Akutsu, M ;
Ishizaki, T ;
Sato, H .
PLANT CELL REPORTS, 2004, 22 (08) :561-568
[4]   Cloning of the chrysanthemum UEP1 promoter and comparative expression in florets and leaves of Dendranthema grandiflora [J].
Annadana, S ;
Beekwilder, MJ ;
Kuipers, G ;
Visser, PB ;
Outchkourov, N ;
Pereira, A ;
Udayakumar, M ;
De Jong, J ;
Jongsma, MA .
TRANSGENIC RESEARCH, 2002, 11 (04) :437-445
[5]   The potato Lhca3.St.1 promoter confers high and stable transgene expression in chrysanthemum, in contrast to CaMV-based promoters [J].
Annadana, S ;
Mlynárová, L ;
Udayakumar, M ;
de Jong, J ;
Nap, JP .
MOLECULAR BREEDING, 2002, 8 (04) :335-344
[6]  
Barton K.A., 2000, U.S. patent, Patent No. [6051757, 6,051,757]
[7]   Resistance to Botrytis cinerea in scented geranium transformed with a gene encoding the antimicrobial protein Ace-AMP1 [J].
Bi, YM ;
Cammue, BPA ;
Goodwin, PH ;
KrishnaRaj, S ;
Saxena, PK .
PLANT CELL REPORTS, 1999, 18 (10) :835-840
[8]   Transgenic regal pelargoniums that express the rolC gene from Agrobacterium rhizogenes exhibit a dwarf floral and vegetative phenotype [J].
Boase, MR ;
Winefield, CS ;
Lill, TA ;
Bendall, MJ .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2004, 40 (01) :46-50
[9]   Long-term expression of the gusA reporter gene in transgenic cyclamen produced from etiolated hypocotyl explants [J].
Boase, MR ;
Marshall, GB ;
Peters, TA ;
Bendall, MJ .
PLANT CELL TISSUE AND ORGAN CULTURE, 2002, 70 (01) :27-39
[10]  
Boase MR, 1998, IN VITRO CELL DEV-PL, V34, P46