THROMBIN HIRUDIN COMPLEX STABILITY - A COMPARISON WITH THE THROMBIN ANTITHROMBIN-III COMPLEX

被引：0
|
作者：
ROMISCH, J
PAQUES, EP
机构：
来源：
BLOOD COAGULATION & FIBRINOLYSIS | 1991年 / 2卷 / 05期
关键词：
THROMBIN-INHIBITOR COMPLEXES; STABILITY; ANTITHROMBIN-III; ATIII HEPARIN; HIRUDIN;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
In the present in vitro study the stabilities of the thrombin-hirudin and the thrombin-antithrombin III complexes were investigated. After incubation of the complexes with free inhibitors the thrombin-antithrombin III levels were determined by ELISA. The thrombin-hirudin complex proved to be stable in the presence of antithrombin III or heparin. However, in the presence of heparin and plasma equivalent concentrations of antithrombin III, the thrombin-hirudin complex dissociated and hirudin was displaced. In contrast, both thrombin-antithrombin III and thrombin-antithrombin III/heparin complexes are very stable even in the presence of a large excess of hirudin.
引用
收藏
页码：643 / 646
页数：4
相关论文
共 50 条
  • [41] ALPHA-THROMBIN WITHIN FIBRIN CLOTS - INACTIVATION OF THROMBIN BY ANTITHROMBIN-III
    NASKI, MC
    SHAFER, JA
    THROMBOSIS RESEARCH, 1993, 69 (05) : 453 - 465
  • [42] HEPARIN-LIKE TUBINGS .3. KINETICS AND MECHANISM OF THROMBIN, ANTITHROMBIN-III AND THROMBIN ANTITHROMBIN COMPLEX ADSORPTION UNDER CONTROLLED-FLOW CONDITIONS
    MIGONNEY, V
    FOUGNOT, C
    JOZEFOWICZ, M
    BIOMATERIALS, 1988, 9 (05) : 413 - 418
  • [43] THROMBIN ANTITHROMBIN-III COMPLEXES ELEVATED IN UNSTABLE ANGINA
    JAFRI, SM
    POLANCO, G
    OZAWA, T
    MAHDYOON, H
    MAMMEN, E
    GHEORGHIADE, M
    GOLDSTEIN, S
    CIRCULATION, 1990, 82 (04) : 277 - 277
  • [44] INTERACTION AND THERMAL-STABILITY OF FLUORESCENT LABELED DERIVATIVES OF THROMBIN AND ANTITHROMBIN-III
    ATHA, DH
    BREW, SA
    INGHAM, KC
    BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 785 (1-2) : 1 - 6
  • [45] DISSOCIATION OF ANTITHROMBIN-III-THROMBIN COMPLEX - FORMATION OF ACTIVE AND INACTIVE ANTI-THROMBIN-III
    GRIFFITH, MJ
    LUNDBLAD, RL
    BIOCHEMISTRY, 1981, 20 (01) : 105 - 110
  • [46] THE COVALENT NATURE OF THE HUMAN ANTITHROMBIN-III THROMBIN BOND
    LONGAS, MO
    FINLAY, TH
    BIOCHEMICAL JOURNAL, 1980, 189 (03) : 481 - 489
  • [47] ALTERED INTERACTION OF THROMBIN AND ANTITHROMBIN-III WITH THE VASCULAR WALL
    BASHKOV, G
    KALISHEVSKAYA, T
    STRUKOVA, S
    THROMBOSIS AND HAEMOSTASIS, 1987, 58 (01) : 150 - 150
  • [48] MODULATIONS OF THROMBIN ACTIVITY BY POLYELECTROLYTES IN THE PRESENCE OF ANTITHROMBIN-III
    SCHIELE, J
    HEUCK, CC
    SEMINARS IN THROMBOSIS AND HEMOSTASIS, 1991, 17 : 35 - 41
  • [49] SPECIFIC BINDING OF THROMBIN-ANTITHROMBIN-III COMPLEX TO HEPATOCYTES
    BAUER, PI
    MANDL, J
    MACHOVICH, R
    ANTONI, F
    GARZO, T
    HORVATH, I
    THROMBOSIS RESEARCH, 1982, 28 (05) : 595 - 606
  • [50] FORMATION, CHARACTERIZATION AND DETECTION OF A TERNARY COMPLEX BETWEEN S-PROTEIN, THROMBIN AND ANTITHROMBIN-III IN SERUM
    PREISSNER, KT
    ZWICKER, L
    MULLERBERGHAUS, G
    BIOCHEMICAL JOURNAL, 1987, 243 (01) : 105 - 111
12345