THROMBIN HIRUDIN COMPLEX STABILITY - A COMPARISON WITH THE THROMBIN ANTITHROMBIN-III COMPLEX

被引：0
|
作者：
ROMISCH, J
PAQUES, EP
机构：
来源：
BLOOD COAGULATION & FIBRINOLYSIS | 1991年 / 2卷 / 05期
关键词：
THROMBIN-INHIBITOR COMPLEXES; STABILITY; ANTITHROMBIN-III; ATIII HEPARIN; HIRUDIN;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
In the present in vitro study the stabilities of the thrombin-hirudin and the thrombin-antithrombin III complexes were investigated. After incubation of the complexes with free inhibitors the thrombin-antithrombin III levels were determined by ELISA. The thrombin-hirudin complex proved to be stable in the presence of antithrombin III or heparin. However, in the presence of heparin and plasma equivalent concentrations of antithrombin III, the thrombin-hirudin complex dissociated and hirudin was displaced. In contrast, both thrombin-antithrombin III and thrombin-antithrombin III/heparin complexes are very stable even in the presence of a large excess of hirudin.
引用
收藏
页码：643 / 646
页数：4
相关论文
共 50 条
  • [1] THROMBIN AND ANTITHROMBIN-III NEO-ANTIGENS IN HUMAN THROMBIN-ANTITHROMBIN-III COMPLEX
    MCDUFFIE, FC
    PETERSON, JM
    CLARK, GW
    OWEN, CA
    MANN, KG
    CIRCULATION, 1978, 58 (04) : 135 - 135
  • [2] INVITRO INHIBITION OF THROMBIN-ANTITHROMBIN-III COMPLEX GENERATION BY HIRUDIN
    HAALAND, A
    SKJONSBERG, OH
    RUYTER, R
    GODAL, HC
    THROMBOSIS AND HAEMOSTASIS, 1989, 62 (01) : 532 - 532
  • [3] THROMBIN ANTITHROMBIN-III COMPLEX LEVELS IN LIVER-CIRRHOSIS
    BAKKER, CM
    DEMAAT, MPM
    KNOT, EAR
    THROMBOSIS AND HAEMOSTASIS, 1989, 62 (01) : 365 - 365
  • [4] THROMBIN ANTITHROMBIN-III COMPLEX ELEVATION SEEN IN ISCHEMIC CARDIOMYOPATHY
    JAFRI, SM
    MAMMEN, E
    BUDZINSKI, D
    FLORES, R
    LEVINE, TB
    CLINICAL RESEARCH, 1989, 37 (04): : A880 - A880
  • [5] DYNAMIC FLUCTUATIONS IN BLOOD OF THROMBIN ANTITHROMBIN-III COMPLEX (TAT)
    DEGUCHI, K
    NOGUCHI, M
    YUWASAKI, E
    ENDOU, T
    DEGUCHI, A
    WADA, H
    MURASHIMA, S
    NISHIKAWA, M
    SHIRAKAWA, S
    TANAKA, K
    KUSAGAWA, M
    AMERICAN JOURNAL OF HEMATOLOGY, 1991, 38 (02) : 86 - 89
  • [6] KINETICS OF FORMATION AND DISSOCIATION OF THE BOVINE THROMBIN - ANTITHROMBIN-III COMPLEX
    JESTY, J
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1979, 254 (20) : 44 - 50
  • [7] DETECTION OF HYPERCOAGULABILITY ASSOCIATED WITH MALIGNANCIES BY DETERMINATION OF THROMBIN ANTITHROMBIN-III COMPLEX
    WAGNER, C
    DATI, F
    BARTHELS, M
    EGBRING, R
    FREUND, M
    NORFOLK, D
    SEITZ, R
    TATRA, G
    JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 1988, 26 (11): : 777 - 778
  • [8] ENZYME-IMMUNOASSAY FOR DETERMINATION OF HUMAN THROMBIN ANTITHROMBIN-III COMPLEX
    PELZER, H
    SCHWARZ, A
    HEIMBURGER, N
    THROMBOSIS RESEARCH, 1986, : 51 - 51
  • [9] THROMBIN-HIRUDIN COMPLEX-FORMATION, THROMBIN-ANTITHROMBIN-III COMPLEX-FORMATION, AND THROMBIN GENERATION AFTER INTRINSIC ACTIVATION OF PLASMA
    GALLISTL, S
    MUNTEAN, W
    THROMBOSIS AND HAEMOSTASIS, 1994, 72 (03) : 387 - 392
  • [10] THE CONFORMATIONAL-CHANGES DURING COMPLEX-FORMATION OF ANTITHROMBIN-III AND THROMBIN OCCUR IN THE ANTITHROMBIN-III MOLECULE
    VILLANUEVA, GB
    ALLEN, N
    THROMBOSIS AND HAEMOSTASIS, 1985, 54 (01) : 24 - 24
12345