INFLUENCE OF SUBSTRATE ON THE AMINO-ACID PROFILE AND MACROMOLECULAR-COMPOSITION OF A BREVIBACTERIUM SPECIES

Brevibacterium JM98A (ATCC 29895) was grown aerobically in C-limited continuous culture on the following substrates: gluconic acid, galactose, fumaric acid, glutamic acid and asparagine. Both whole and trichloroacetic acid(TCA)-extracted cells were analyzed for their amino acid compositions. No significant variations of amino acid profile were induced by change of substrate. Only the valine content varied significantly with growth rate. Some significant variations were observed between whole and extracted cell samples, primarily in the levels of the essential amino acids threonine, cystine and valine.