STABLE INTEGRATIVE TRANSFORMATION OF TRYPANOSOMA-BRUCEI THAT OCCURS EXCLUSIVELY BY HOMOLOGOUS RECOMBINATION

被引：91

作者：

EID, J

SOLLNERWEBB, B

机构：

[1] Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205-2185

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 06期

关键词：

GENE TARGETING; TRANSFECTION; CALMODULIN GENE; NEOMYCIN-RESISTANCE GENE; HOMOLOGOUS INTEGRATION;

D O I：

10.1073/pnas.88.6.2118

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A calmodulin-neomycin-resistance fusion gene was introduced into Trypanosoma brucei by electroporation, and stably transformed cell lines were obtained. In all of the transformants, the fusion gene had integrated into the host genome at the cognate locus, evidently by homologous recombination within flanking calmodulin DNA. This unusual observation distinguishes trypanosomes as the only eukaryote other than yeast known to undergo gene targeting in essentially 100% of the stable transformants. It should now be possible to systematically manipulate the trypanosome genome, directing predetermined mutations to virtually any chromosomal locus.

引用

页码：2118 / 2121

页数：4

共 35 条

[1]

ASHBROEK AL, 1990, NATURE, V348, P174

[2]

AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303

[3]

Ausubel FM., 1988, CURRENT PROTOCOLS MO

[4] NUCLEOTIDE-SEQUENCE AND EXACT LOCALIZATION OF THE NEOMYCIN PHOSPHOTRANSFERASE GENE FROM TRANSPOSON TN5 [J].