MOLECULAR WEIGHT DETERMINATION BY POLYACRYLAMIDE GEL ELECTROPHORESIS WITH A LINEAR GEL GRADIENT

被引：90
作者：
KOPPERSCHLAGER, G
DIEZEL, W
BIERWAGE.B
HOFMANN, E
机构：
[1] Physiologisch-chemisches Institut der Karl-Marx-Universität, Leipzig
来源：
FEBS LETTERS | 1969年 / 5卷 / 03期
关键词：
D O I：
10.1016/0014-5793(69)80337-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method for molecular weight determination using polyacrylamidegel electrophoresis in a linear gel-concentration-gradient (3-20°) is described. Plotting the log of molecular weights of several standard proteins against distance of migration or against 3 log of rate of n-dgration reveal linear relationships in ranges of 50.000 - 200.000 or 100.000 - 400.000 Daltons respectively. On this basis, a simple method for molecular weight determination of proteins (accuracy ± 5°) has been devised. The method can also be applied for an individual protein in protein mixtures using specific staining procedures. © 1969.
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页码：221 / +
页数：1
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