ANGIOTENSIN-II INDUCES CALCIUM RELEASE IN A SUBPOPULATION OF SINGLE OVARIAN (GRANULOSA) CELLS

被引：18

作者：

FLORES, JA

VELDHUIS, JD

LEONG, DA

机构：

[1] UNIV VIRGINIA, HLTH SCI CTR,DEPT INTERNAL MED,DIV ENDOCRINOL, NSF SCI CTR BIOL TIMING,BOX 202, CHARLOTTESVILLE, VA 22908 USA

[2] UNIV VIRGINIA, HLTH SCI CTR, DEPT MED, CHARLOTTESVILLE, VA 22908 USA

[3] UNIV VIRGINIA, HLTH SCI CTR, DEPT NEUROSCI, CHARLOTTESVILLE, VA 22908 USA

[4] UNIV VIRGINIA, HLTH SCI CTR, DEPT PHYSIOL, CHARLOTTESVILLE, VA 22908 USA

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1991年 / 81卷 / 1-3期

关键词：

CALCIUM RELEASE; GRANULOSA CELL; ANGIOTENSIN-II;

D O I：

10.1016/0303-7207(91)90199-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effects of angiotensin II on cytosolic free Ca2+ ion concentrations ([Ca2+]i) were studied in single porcine granulosa cells using the calcium-sensitive fluorescent dye fura-2 and high temporal resolution fluorescent videomicroscopy. Angiotensin II initiated specific, rapid, transient and topographically organized increases in [Ca2+]i in a subpopulation of single swine granulosa cells. The Ca2+ source for this angiotensin II-mediated [Ca2+]i transient appeared to be internal stores, and a pertussis toxin-sensitive guanine nucleotide binding protein was implicated in this receptor-mediated Ca2+ rise. Our single-cell studies also revealed a striking functional heterogeneity among granulosa cells, since follicle-stimulating hormone-responsive cells were not angiotensin II responsive. We conclude that single swine granulosa cells are targets of specific angiotensin II action on intracellular pools of Ca2+.

引用

页码：1 / 10

页数：10

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